Barrett A J, Faille A, Ketels F
Br J Haematol. 1979 Jul;42(3):337-44. doi: 10.1111/j.1365-2141.1979.tb01141.x.
Blood granulocyte colony forming units (CFU-C) were studied in normal adults to establish: (i) a normal range; (ii) variability due to the culture technique; (iii) variability of blood CFU-C within individuals. Thirty men studied had 98 (range 8--300) CFU-C X 10(3)/1, and 28 women studied had 44 (range 0--260) CFU-C X 10(3)/1. This difference was significant (P less than 0.001). There was also a significant sex difference in the total number of cells forming colonies and clusters per litre; and in the incidence of colony formers and of cluster formers in buffy coat and mononuclear cell blood fractions. CFU-C were assayed in four subjects over a 10 week period. When buffy coat cells were used as a source of colony stimulation the week to week variation in the combined growth of the four subjects was wide (+/- 36%) but with conditioned medium the variation was smaller (+/- 14%). In all subjects colony and cluster growth varied in the same way (r = 0.77, P = .001) but there was no correlation with the total leucocyte count. A 3--4 week cyclical change in CFU-C/1 was found which was independent of the variation inherent in the technique. The physiological significance of the sex difference and the apparent cyclical changes in blood CFU-C are not explained, but the results emphasize the wide fluctuations in CFU-C that may occur in normal individuals.
对正常成年人的血液粒细胞集落形成单位（CFU-C）进行了研究，以确定：（i）正常范围；（ii）培养技术导致的变异性；（iii）个体内血液CFU-C的变异性。研究的30名男性每升血液中有98（范围8 - 300）×10³个CFU-C，28名女性每升血液中有44（范围0 - 260）×10³个CFU-C。这种差异具有显著性（P小于0.001）。每升形成集落和集簇的细胞总数以及白膜层和单核细胞血液组分中集落形成细胞和集簇形成细胞的发生率也存在显著的性别差异。在10周的时间里对4名受试者的CFU-C进行了检测。当使用白膜层细胞作为集落刺激源时，4名受试者的联合生长每周变化很大（±36%），但使用条件培养基时变化较小（±14%）。在所有受试者中，集落和集簇的生长变化方式相同（r = 0.77，P = 0.001），但与白细胞总数无关。发现每升CFU-C存在3 - 4周的周期性变化，这与该技术固有的变异性无关。性别差异以及血液CFU-C明显的周期性变化的生理意义尚不清楚，但结果强调了正常个体中CFU-C可能出现的广泛波动。
