Wuepper K D
J Exp Med. 1973 Dec 1;138(6):1345-55. doi: 10.1084/jem.138.6.1345.
Blood plasma obtained from an individual with abnormal thromboplastin formation, due to deficiency of Fletcher factor, was fully corrected by 2% of normal, Hageman factor- or PTA-deficient plasma. It was also reconstituted by addition of highly purified human or rabbit prekallikrein. The plasma failed to generate kinin upon exposure to kaolin, a defect which was also corrected by addition of prekallikrein. Prekallikrein antigen was not detectable in this plasma. Fletcher factor-deficient plasma did not support the normal generation of PF/dil when dilute plasma was incubated in glass vessels and injected intracutaneously. Small quantities of Fletcher factor-deficient or Hageman factor-deficient plasma corrected the ability of the other to generate PF/dil. The formation of plasmin in dilute, acidified plasma incubated with kaolin was also abnormal in Fletcher factor-deficient plasma. Plasmin generation was normalized by addition of prekallikrein or small quantities of Hageman factor-deficient plasma. The data support the identity of Fletcher factor and prekallikrein.
从因弗勒彻因子缺乏而导致凝血活酶生成异常的个体获取的血浆，可被2%的正常血浆、哈格曼因子或血浆促凝血酶原激酶（PTA）缺乏的血浆完全纠正。通过添加高度纯化的人或兔前激肽释放酶也可使其恢复。该血浆在接触高岭土时无法生成激肽，添加前激肽释放酶也可纠正这一缺陷。在此血浆中检测不到前激肽释放酶抗原。当稀释血浆在玻璃容器中孵育并皮内注射时，弗勒彻因子缺乏的血浆不支持正常生成PF/dil。少量弗勒彻因子缺乏或哈格曼因子缺乏的血浆可纠正另一方生成PF/dil的能力。在与高岭土孵育的稀释酸化血浆中，弗勒彻因子缺乏的血浆中纤溶酶的形成也异常。通过添加前激肽释放酶或少量哈格曼因子缺乏的血浆可使纤溶酶生成正常化。这些数据支持弗勒彻因子与前激肽释放酶的一致性。
