Morardet N, Charbord P, Parmentier C
Biomedicine. 1979 Feb;31(1):4-5.
A suicide technique applicable to human bone marrow granulocytic progenitor cells has been previously described (3). This study discusses the conditions for its use on bone marrow aspirates poor in cells. When the number of cells exposed to hydroxyurea (HU) is proportional to the quantity of HU it is possible to avoid artifacts due to HU toxicity. This technique can repeatedly reproduce a 40--50% suicide. To obtain this result we suggest using a 6.10(-6) mM/ml/cell HU concentration.
一种适用于人类骨髓粒细胞祖细胞的自杀技术先前已有描述(3)。本研究讨论了在细胞数量较少的骨髓抽吸物上使用该技术的条件。当暴露于羟基脲(HU)的细胞数量与HU的量成比例时，有可能避免由于HU毒性导致的假象。该技术可反复产生40%-50%的自杀率。为获得这一结果，我们建议使用6×10⁻⁶ mM/ml/细胞的HU浓度。
