Mellstedt H, Holm G
Clin Exp Immunol. 1973 Nov;15(3):309-20.
Highly purified blood lymphocytes from patients with plasma cell myeloma were tested in different systems. The patients were untreated or had received a standardized 4-day treatment with melphalan and prednisolone every sixth week. They were tested 5 weeks after the last treatment to minimize the acute toxic effects of the drugs. Lymphocytes from healthy controls were included in each experiment. Stimulation of lymphocytes was measured by incorporation of C-thymidine into DNA after activation with phytohaemagglutinin (PHA), concancavalin A (Con A) and pokeweed mitogen (PWM). Their cytotoxicity was tested against Chang cells (human cell line) and chicken red blood cells in presence of PHA or heat-inactivated rabbit antibodies to target cell antigens. Lysis was quantitated as release of radio-activity from target cells labelled with Cr-chromate. Antibody-induced cytotoxicity of lymphocytes from untreated patients was normal or slightly elevated while that of treated patients was severely depressed. Also, lymphocytes from treated patients were significantly less stimulated to DNA synthesis by PWM than were control lymphocytes. PHA-induced cytotoxicity and stimulation of lymphocytes by Con A or PHA were normal in all groups. These results suggest that treatment of myeloma patients with melphalan and cortisone selectively impairs lymphocytes which respond to PWM by DNA synthesis and which participate in antibody-mediated cytolysis.
对浆细胞骨髓瘤患者的高度纯化的血液淋巴细胞在不同系统中进行了检测。这些患者未接受治疗或每六周接受一次美法仑和泼尼松龙的标准化4天治疗。在最后一次治疗后5周对他们进行检测，以尽量减少药物的急性毒性作用。每次实验都纳入了健康对照者的淋巴细胞。在用植物血凝素（PHA）、刀豆球蛋白A（Con A）和商陆有丝分裂原（PWM）激活后，通过将C-胸腺嘧啶核苷掺入DNA来测量淋巴细胞的刺激情况。在PHA或针对靶细胞抗原的热灭活兔抗体存在的情况下，测试它们对Chang细胞（人细胞系）和鸡红细胞的细胞毒性。裂解情况通过用铬酸铬标记的靶细胞释放放射性来定量。未治疗患者淋巴细胞的抗体诱导细胞毒性正常或略有升高，而治疗患者的则严重降低。此外，与对照淋巴细胞相比，治疗患者的淋巴细胞受PWM刺激后DNA合成明显减少。所有组中PHA诱导的细胞毒性以及Con A或PHA对淋巴细胞的刺激均正常。这些结果表明，用美法仑和可的松治疗骨髓瘤患者会选择性损害那些通过DNA合成对PWM作出反应并参与抗体介导细胞溶解的淋巴细胞。
