Holm G, Perlmann P, Johansson B
Clin Exp Immunol. 1967 May;2(3):351-60.
Lymphocytes from healthy, unsensitized donors damage allogeneic tissue culture target cells in the presence of phytohaemagglutinin (PHA). In this investigation, blood lymphocytes from eleven patients with clinically active Hodgkin's disease and from eight patients with chronic lymphatic leukaemia (CLL) were tested for PHA-induced cytotoxicity to Chang cells. Blastoid transformation and DNA-synthesis in the presence of PHA were studied in parallel experiments. The cytotoxicity of the patients' lymphocytes was significantly lower than that of lymphocytes from healthy donors. Synthesis of DNA and blastoid transformation was subnormal in all cases of CLL and in most cases with Hodgkin's disease. A good correlation between cytotoxicity and stimulation by PHA was observed in most cases. The incidence of positive skin reactions to tuberculin was high in the CLL-group and low in the Hodgkin group. If it is assumed that the pool of immunologically competent cells was normal in the CLL-patients, this would account for their intact cellular reactivity . In contrast, the high proportion of leukaemic cells could be expected to mask the reactivity of the normal CLL-lymphocytes . The weak reactivity of the lymphocytes in Hodgkin's disease is in line with the view that deficient lymphocytes are present in this disease and suggests that the patients' pool of immunologically competent cells is reduced. This could also contribute to the anergic state seen in these patients.
来自健康、未致敏供体的淋巴细胞在植物血凝素（PHA）存在的情况下会破坏同种异体组织培养靶细胞。在本研究中，对11例临床活动期霍奇金病患者和8例慢性淋巴细胞白血病（CLL）患者的血液淋巴细胞进行了PHA诱导的对Chang细胞的细胞毒性测试。在平行实验中研究了PHA存在下的母细胞样转化和DNA合成。患者淋巴细胞的细胞毒性明显低于健康供体的淋巴细胞。在所有CLL病例和大多数霍奇金病病例中，DNA合成和母细胞样转化均低于正常水平。在大多数情况下，观察到细胞毒性与PHA刺激之间有良好的相关性。CLL组对结核菌素的皮肤阳性反应发生率高，而霍奇金组低。如果假设CLL患者免疫活性细胞库正常，这可以解释他们完整的细胞反应性。相反，白血病细胞的高比例可能会掩盖正常CLL淋巴细胞的反应性。霍奇金病中淋巴细胞的弱反应性与该疾病中存在缺陷淋巴细胞的观点一致，并表明患者的免疫活性细胞库减少。这也可能导致这些患者出现无反应状态。
