Sweet M B, Thonar E J, Berson S D, Skikne M I, Immelman A R, Kerr W A
Cancer. 1979 Aug;44(2):652-60. doi: 10.1002/1097-0142(197908)44:2<652::aid-cncr2820440235>3.0.co;2-2.
Glycosaminoglycans of human chordoma and a metastasis were studied. The glycosaminoglycans were derived from the extracellular matrix and consisted of chondroitin 4- and 6-sulphate, keratan sulphate and hyaluronate. The ratio of chondroitin sulphate to keratan sulphate was much lower in the metastasis than in the primary. Proteoglycan extracted with 4M guanidinium chloride and purified by associative density-gradient centrifugation was assessed on Sepharose 2B before and after reduction and alkylation. The result of this experiment suggested that only a small proportion of the proteoglycans were aggregated.
对人脊索瘤及其转移灶的糖胺聚糖进行了研究。这些糖胺聚糖来源于细胞外基质，由硫酸软骨素4-硫酸盐、硫酸软骨素6-硫酸盐、硫酸角质素和透明质酸盐组成。转移灶中硫酸软骨素与硫酸角质素的比例远低于原发灶。用4M氯化胍提取并用关联密度梯度离心法纯化的蛋白聚糖，在还原和烷基化前后在琼脂糖2B上进行评估。该实验结果表明，只有一小部分蛋白聚糖发生了聚集。
