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对从霍乱弧菌稻叶569B分离出的脂多糖的结构研究。

Structural investigations of the lipopolysaccharide isolated from Vibrio cholera, Inaba 569 B.

作者信息

Sen A K, Mukherjee A K, Guhathakurta B, Dutta A, Sasmal D

出版信息

Carbohydr Res. 1979 Jul;72:191-99. doi: 10.1016/s0008-6215(00)83935-x.

DOI:10.1016/s0008-6215(00)83935-x
PMID:476718
Abstract

On hydrolysis, the purified lipopolysaccharide (LPS) isolated from Vibrio cholera, Inaba 569 B, yielded glucose, mannose, a heptose behaving like D-glycero-L-manno-heptose and one behaving like D-glycero-L-gluco-heptose, 2-amino-2-deoxyglucose, and glucuronic acid in the molar ratios of approximately 9:4:5:1:2:5. Studies on the LPS, the polysaccharide (PS), and carboxyl-reduced LPS showed that the PS has a branched structure, with (1 leads to 2)-linked mannopyranosyl and a heptopyranosyl, and (1 leads to 4)-linked glucopyranosyluronic and 2-amino-2-deoxyglucopyranosyl residues in the interior part of the molecule, and glucopyranosyl and heptopyranosyl residues as nonreducing end-groups.

摘要

从霍乱弧菌稻叶569B中分离得到的纯化脂多糖(LPS)水解后,产生葡萄糖、甘露糖、一种表现得像D-甘油-L-甘露庚糖的庚糖和一种表现得像D-甘油-L-葡糖庚糖的庚糖、2-氨基-2-脱氧葡萄糖和葡萄糖醛酸,其摩尔比约为9:4:5:1:2:5。对LPS、多糖(PS)和羧基还原LPS的研究表明,PS具有分支结构,在分子内部有(1→2)连接的甘露吡喃糖基和一个庚吡喃糖基,以及(1→4)连接的葡萄糖醛酸吡喃糖基和2-氨基-2-脱氧葡萄糖醛酸吡喃糖基残基,葡萄糖吡喃糖基和庚吡喃糖基残基作为非还原端基。

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引用本文的文献

1
Characterization of the lipopolysaccharide from Vibrio cholerae 395 (Ogawa).霍乱弧菌395(小川型)脂多糖的特性分析。
Infect Immun. 1982 Dec;38(3):1263-72. doi: 10.1128/iai.38.3.1263-1272.1982.
2
Characterization of the Shigella serotype D (S. sonnei) O polysaccharide and the enterobacterial R1 lipopolysaccharide core by use of mouse monoclonal antibodies.利用小鼠单克隆抗体对痢疾志贺氏菌血清型D(宋内志贺氏菌)O多糖和肠杆菌R1脂多糖核心进行表征。
Infect Immun. 1992 Jul;60(7):2741-7. doi: 10.1128/iai.60.7.2741-2747.1992.