Kaca W, Brade L, Rietschel E T, Brade H
Carbohydr Res. 1986 Jul 1;149(2):293-8. doi: 10.1016/s0008-6215(00)90052-1.
The lipopolysaccharide (LPS) of a rough mutant (95R) of Vibrio cholerae Ogawa has been investigated chemically and serologically. D-Fructose was released from LPS under conditions (10mM trifluoroacetic acid, 60 degrees, 1 h) that liberated no other sugar constituent of the LPS (2-amino-2-deoxy-D-glucose, D-glucose, L-glycero-D-manno-heptose). Upon periodate oxidation, D-fructose and D-glucose were oxidised quantitatively, whereas approximately 50% of heptose was periodate-resistant. The data indicate that D-fructose does not link the polysaccharide and lipid A portion as proposed earlier, and suggest that D-fructose is present as a branch. By passive hemolysis inhibition, it was shown that the release of D-fructose paralleled the exposure of an antigenic determinant cryptic in LPS.
对霍乱弧菌小川型粗糙突变体（95R）的脂多糖（LPS）进行了化学和血清学研究。在不会释放LPS其他糖成分（2-氨基-2-脱氧-D-葡萄糖、D-葡萄糖、L-甘油-D-甘露庚糖）的条件下（10mM三氟乙酸，60℃，1小时），从LPS中释放出了D-果糖。经高碘酸盐氧化后，D-果糖和D-葡萄糖被定量氧化，而约50%的庚糖对高碘酸盐具有抗性。数据表明，D-果糖并非如先前提出的那样连接多糖和脂质A部分，而是表明D-果糖以分支形式存在。通过被动溶血抑制试验表明，D-果糖的释放与LPS中隐蔽抗原决定簇的暴露情况平行。
