Tollinger C D, Vreman H J, Weiner M W
Clin Chem. 1979 Oct;25(10):1787-90.
We measured acetate concentrations in whole blood, serum, and plasma by a modification of a previously described method involving vacuum distillation and gas chromatography. The mean acetate concentration of fresh venous plasma from 27 normal subjects was 51 +/- 5 mumol/L (95% confidence limits ranged from 0 to 103 mumol/L). The acetate concentrations of serum and plasma incubated for 2 h at either 4 degrees C or 27 degrees C were the same. The acetate concentration of whole blood incubated at 27 degrees C was significantly greater than that of blood incubated at 4 degrees C. This change may have resulted from the production of acetate by erythrocytes or from the hydrolysis of acetate esters. Storage of plasma at -20 degrees C for 24 h significantly increased acetate concentrations from 26 +/- 6 mumol/L to 63 +/- 4 mumol/L. After the subjects consumed a standard breakfast, venous plasma acetate concentrations increased from 58 to 97 mumol/L at 30 min. Acetate concentrations in arterial plasma exceeded those in venous plasma. Plasma acetate concentrations were not significantly altered in patients with malignancy or diabetes mellitus, but severe liver disease and severe acidosis were both associated with increased acetate concentrations. These preliminary observations suggest that plasma acetate concentrations may be altered in several disease states.
我们通过对先前描述的涉及真空蒸馏和气相色谱法的方法进行改进，来测量全血、血清和血浆中的乙酸盐浓度。27名正常受试者新鲜静脉血浆中乙酸盐的平均浓度为51±5μmol/L（95%置信区间为0至103μmol/L）。在4℃或27℃下孵育2小时的血清和血浆中的乙酸盐浓度相同。在27℃下孵育的全血中的乙酸盐浓度显著高于在4℃下孵育的血液中的乙酸盐浓度。这种变化可能是由于红细胞产生乙酸盐或乙酸酯水解所致。血浆在-20℃下储存24小时后，乙酸盐浓度从26±6μmol/L显著增加至63±4μmol/L。受试者食用标准早餐后，静脉血浆乙酸盐浓度在30分钟时从58μmol/L增加至97μmol/L。动脉血浆中的乙酸盐浓度超过静脉血浆中的浓度。恶性肿瘤或糖尿病患者的血浆乙酸盐浓度无显著变化，但严重肝病和严重酸中毒均与乙酸盐浓度升高有关。这些初步观察结果表明，在几种疾病状态下血浆乙酸盐浓度可能会发生改变。
