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用于醋酸不耐受研究中血浆醋酸盐分析的气相色谱法。

Gas-chromatographic method for plasma acetate analysis in acetate-intolerance studies.

作者信息

Nielsen L G, Ash K O, Thor E

出版信息

Clin Chem. 1978 Feb;24(2):348-50.

PMID:627069
Abstract

We describe a modified gas-chromatographic method for acetate in serum or plasma, intended for use in the investigation of acetate intolerance in hemodialysis patients. The assay may be adapted for use with a single-column gas chromatograph equipped with a flame ionization detector. The analysis, made isothermally, requires only 0.5 ml of plasma or serum. Only one deproteinizing step is required to prepare the sample for analysis. Additionally, we present preliminary findings of an ongoing acetate-intolerance study.

摘要

我们描述了一种用于血清或血浆中醋酸盐的改良气相色谱法,旨在用于调查血液透析患者的醋酸盐不耐受情况。该测定法可适用于配备火焰离子化检测器的单柱气相色谱仪。等温进行的分析仅需0.5毫升血浆或血清。制备样品进行分析仅需一个脱蛋白步骤。此外,我们还展示了一项正在进行的醋酸盐不耐受研究的初步结果。

相似文献

1
Gas-chromatographic method for plasma acetate analysis in acetate-intolerance studies.用于醋酸不耐受研究中血浆醋酸盐分析的气相色谱法。
Clin Chem. 1978 Feb;24(2):348-50.
2
[Vapor-phase gas chromatographic determination of acetate in the blood].[血液中乙酸盐的气相色谱测定法]
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Urol Nefrol (Mosk). 1990 Nov-Dec(6):39-44.
4
Correction of metabolic acidosis and changes in plasma acetate levels in acetate and bicarbonate dialyses and acetate-free biofiltration.
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Acetate vs. bicarbonate dialysis.
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[Rapid gas chromatographic method for determination of acetate in human plasma and hemodialysis baths (author's transl)].[快速气相色谱法测定人血浆和血液透析液中的醋酸盐(作者译)]
Clin Chim Acta. 1977 Apr 15;76(2):193-204. doi: 10.1016/0009-8981(77)90096-1.
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Improved effect of hemodialysis on acidemic patients from an acetate concentration of 38 mmol/l.醋酸盐浓度为38 mmol/l时,血液透析对酸血症患者的疗效改善。
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[Plasma acetate and bicarbonate kinetics and intolerance to acetate during and after renal dialysis].[肾透析期间及之后血浆乙酸盐和碳酸氢盐动力学及对乙酸盐的不耐受性]
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Acetate intolerance and an inhibitor of acetate utilization in hemodialysis patients.血液透析患者的醋酸盐不耐受及醋酸盐利用抑制剂
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Measurement of acetate in human blood by gas chromatography: effects of sample preparation, feeding, and various diseases.气相色谱法测定人血中的乙酸:样品制备、饮食及各种疾病的影响
Clin Chem. 1979 Oct;25(10):1787-90.