Verch R L, Wallach S, Peabody R A
Clin Chim Acta. 1979 Aug 15;96(1-2):125-30. doi: 10.1016/0009-8981(79)90061-5.
A modified urinary hydroxyproline method is presented which utilizes an automated technique, Technicon instrumentation and Ehrlich's reagent. Acid hydrolysates are neutralized automatically. Non-specific reacting substances are determined simultaneously with each sample and subtracted as a blank. This method has been found to be superior to the use of charcoal-resin as an absorbent. After blank correction, the mean hydroxyproline excretion of normal and osteoporotic patients are similar. In all groups studied, including Paget's disease, the blanks reresent a significant fraction of total reacting substances and show wide variation between samples. Multiple samples from patients over a two year period show little variation in hydroxyproline excretion when corrected for non-specific reacting substances.
本文介绍了一种改良的尿羟脯氨酸检测方法，该方法采用自动化技术、Technicon仪器和艾氏试剂。酸性水解产物可自动中和。与每个样本同时测定非特异性反应物质，并作为空白值减去。已发现该方法优于使用活性炭树脂作为吸附剂。经过空白校正后，正常患者和骨质疏松患者的平均羟脯氨酸排泄量相似。在所有研究组中，包括佩吉特病，空白值占总反应物质的很大一部分，且样本间差异很大。对患者在两年期间采集的多个样本进行非特异性反应物质校正后，羟脯氨酸排泄量几乎没有变化。
