一种测定尿液和血浆中乙醇酸盐的新酶法。

A new enzymatic method for the determination of glycollate in urine and plasma.

作者信息

Kasidas G P, Rose G A

出版信息

Clin Chim Acta. 1979 Aug 15;96(1-2):25-36. doi: 10.1016/0009-8981(79)90048-2.

Abstract

An enzymatic assay for the measurement of glycollate in urine and plasma is described. Gycollic acid oxidase (glycollate:oxygen oxidoreductase, EC 1.1.3), extracted from spinach leaves, is used in the enzymatic oxidation of glycollate to produce glyoxylate and hydrogen peroxide. In the presence of peroxidase, the hydrogen peroxide oxidatively couples sulphonated 2,4-dichlorophenol and 4-aminophenazone to form a soluble purple quinoneimine dye. Interfering substances are removed from plasma by deproteinisation and from urine by adsorption onto activated charcoal before analysis. Glycollic acid oxidase also catalyses the oxidation of lactic acid which therefore has to be determined separately. The mean normal urinary glycollate for adults is 0.19 mmol/24 h with no difference between males and females. The mean normal plasma glycollate for adults is 0.17 mmol/l, but higher in males than in females.

摘要

本文描述了一种用于测量尿液和血浆中乙醇酸盐的酶促测定法。从菠菜叶中提取的乙醇酸氧化酶（乙醇酸盐：氧氧化还原酶，EC 1.1.3）用于乙醇酸盐的酶促氧化，生成乙醛酸和过氧化氢。在过氧化物酶存在的情况下，过氧化氢将磺化的2,4-二氯苯酚和4-氨基苯甲酰偶联氧化，形成可溶性紫色醌亚胺染料。分析前，通过脱蛋白从血浆中去除干扰物质，通过活性炭吸附从尿液中去除干扰物质。乙醇酸氧化酶还催化乳酸的氧化，因此必须单独测定乳酸。成年人正常尿液中乙醇酸盐的平均值为0.19 mmol/24 h，男性和女性之间无差异。成年人正常血浆中乙醇酸盐的平均值为0.17 mmol/l，但男性高于女性。