Speight A C, Hancock K W, Oakey R E
Clin Endocrinol (Oxf). 1979 Apr;10(4):329-41. doi: 10.1111/j.1365-2265.1979.tb02088.x.
Unconjugated oestrone and unconjugated oestradiol-17 beta were measured in 24 h urine specimens from twenty normal men. The concentrations of non-protein-bound oestrone and non-protein-bound oestradiol-17 beta, in samples of blood obtained from these subjects during the time in which the urine was collected, were measured by equilibrium dialysis (using a correction for plasma dilution). The mean excretion of unconjugated oestrone (0.44+/- 0.36 nmol/24 h, mean +/- SD) was significantly greater (Pless than 0.01) than that of unconjugated oestradiol-17 beta (0.20 +/- 0.13 nmol/24 h). The concentrations of non-protein-bound oestrone and non-protein-bound oestradiol-17 beta in plasma were 5.52 +/- 2.69pmol/1 and 2.42 +/- 0.73 pmol/1, respectively. There was no correlation between the quantity of unconjugated oestrone excreted and the concentration of non-protein-bound oestrone in plasma (r=0.27) nor between the quantity of unconjugated oestradiol-17 beta in the urine and the concentration of non-protein-bound oestradiol-17 beta in plasma (r=0.05). Therefore in normal men, estimation of unconjugated oestrone or oestradiol-17 beta in urine provides no guide to the concentration of the corresponding non-protein-bound oestrogen in plasma.
对20名正常男性24小时尿液样本中的未结合雌酮和未结合雌二醇-17β进行了测定。通过平衡透析法（采用血浆稀释校正）测定了在收集尿液期间从这些受试者采集的血样中未结合蛋白的雌酮和未结合蛋白的雌二醇-17β的浓度。未结合雌酮的平均排泄量（0.44±0.36 nmol/24小时，均值±标准差）显著高于未结合雌二醇-17β（0.20±0.13 nmol/24小时）（P<0.01）。血浆中未结合蛋白的雌酮和未结合蛋白的雌二醇-17β的浓度分别为5.52±2.69 pmol/L和2.42±0.73 pmol/L。尿液中未结合雌酮的排泄量与血浆中未结合蛋白的雌酮浓度之间无相关性（r=0.27），尿液中未结合雌二醇-17β的量与血浆中未结合蛋白的雌二醇-17β浓度之间也无相关性（r=0.05）。因此，对于正常男性，尿液中未结合雌酮或雌二醇-17β的测定无法为血浆中相应的未结合蛋白雌激素浓度提供指导。
