De Narbonne J M, Thiery J P, Magdelenat H, Mazabraud A
Bull Cancer. 1977;64(1):31-6.
Starting from the fact that an ultrastructural cytochemical study of the Giant-Cell Tumour demonstrated the presence of a large amount of lysosomic acid phosphatase in the tumour cells, an attempt was made to evidence the presence of this enzyme in the serum of patients bearing such a type of bone tumor. Acrylamide gel electrophoresis of the serum allowed one to separate a number of isoenzyme with acid phosphatase activity and to characterize at least two bands different from those secreted by prostate, blood platelets, liver or spleen. The comparison between zymograms of normal and pathological sera, more particularly in Paget disease, led to consider that these two bands had an osteoclastic origin. Besides, these bands vanish after tumor eradication. Acrylamide gel electrophoresis of serum provides therefore a means to support the preoperative diagnosis of Giant-Cell Tumor and, eventually, to detect an early recurrence of the disease.
基于对巨细胞瘤的超微结构细胞化学研究表明肿瘤细胞中存在大量溶酶体酸性磷酸酶这一事实，人们尝试证明患有此类骨肿瘤患者的血清中存在这种酶。血清的丙烯酰胺凝胶电泳能够分离出多种具有酸性磷酸酶活性的同工酶，并鉴定出至少两条与前列腺、血小板、肝脏或脾脏分泌的条带不同的条带。正常血清和病理血清（尤其是佩吉特病患者的血清）酶谱的比较表明，这两条带起源于破骨细胞。此外，肿瘤切除后这些条带消失。因此，血清的丙烯酰胺凝胶电泳为支持巨细胞瘤的术前诊断以及最终检测疾病的早期复发提供了一种方法。
