绵羊肝脏酶体外合成蝶酰多聚谷氨酸。
The synthesis of pteroylpolyglutamates by sheep liver enzymes in vitro.
作者信息
Gawthorne J M, Smith R M
出版信息
Biochem J. 1973 Oct;136(2):295-301. doi: 10.1042/bj1360295.
Abstract
- Sephadex G-15 was used to separate pteroylmonoglutamates from corresponding polyglutamate derivatives. 2. Pteroylpolyglutamates were formed when 5-formyltetrahydro[2-(14)C]pteroylglutamic acid, 5-[methyl-(14)C]tetrahydropteroylglutamic acid or tetrahydro[2-(14)C]pteroylglutamic acid was incubated at pH8.4 with ATP, MgCl(2), KCl, l-glutamic acid and sheep liver cytosol. The gamma-glutamyl side chain appeared to be lengthened by the stepwise addition of single glutamate moieties. 3. The subcellular distribution of pteroylpolyglutamates paralleled that of pteroylpolyglutamate synthetase activity, and followed the order cytosol>;nuclear' fraction>microsomal fraction>mitochondria.
摘要
- 用葡聚糖凝胶G - 15从相应的多聚谷氨酸衍生物中分离蝶酰单谷氨酸。2. 当5 - 甲酰四氢[2 - (14)C]蝶酰谷氨酸、5 - [甲基 - (14)C]四氢蝶酰谷氨酸或四氢[2 - (14)C]蝶酰谷氨酸在pH8.4条件下与ATP、MgCl₂、KCl、L - 谷氨酸和羊肝细胞溶胶一起温育时，会形成蝶酰多聚谷氨酸。γ - 谷氨酰侧链似乎是通过逐个添加单个谷氨酸部分而延长的。3. 蝶酰多聚谷氨酸的亚细胞分布与蝶酰多聚谷氨酸合成酶活性的分布平行，顺序为细胞溶胶＞核部分＞微粒体部分＞线粒体。
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