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在经环氧乙烷灭菌的聚碳酸酯培养瓶中生长对L5178Y小鼠淋巴瘤细胞的诱变作用。

Mutagenic effect on L5178Y mouse lymphoma cells by growth in ethylene oxide-sterilized polycarbonate flasks.

作者信息

Krell K, Jacobson E D, Selby K

出版信息

In Vitro. 1979 May;15(5):326-8. doi: 10.1007/BF02616138.

DOI:10.1007/BF02616138
PMID:478562
Abstract

The mutation frequency of L5178Y mouse lymphoma cells to resistance to 5'-bromo-2'-deoxyuridine increased 6- to 14-fold after growth in ethylene oxide-sterilized polycarbonate culture flasks compared to growth in glass flasks. No comparable increase was observed when L5178Y cells were grown in identical polycarbonate culture flasks sterilized by autoclaving.

摘要

与在玻璃培养瓶中生长相比,L5178Y小鼠淋巴瘤细胞对5'-溴-2'-脱氧尿苷产生抗性的突变频率在环氧乙烷灭菌的聚碳酸酯培养瓶中生长后增加了6至14倍。当L5178Y细胞在通过高压灭菌法灭菌的相同聚碳酸酯培养瓶中生长时,未观察到类似的增加。

相似文献

1
Mutagenic effect on L5178Y mouse lymphoma cells by growth in ethylene oxide-sterilized polycarbonate flasks.在经环氧乙烷灭菌的聚碳酸酯培养瓶中生长对L5178Y小鼠淋巴瘤细胞的诱变作用。
In Vitro. 1979 May;15(5):326-8. doi: 10.1007/BF02616138.
2
Elimination of toxicity from polyvinyl trays after sterilization with ethylene oxide.环氧乙烷灭菌后聚乙烯托盘毒性的消除。
Appl Microbiol. 1969 Dec;18(6):1057-9. doi: 10.1128/am.18.6.1057-1059.1969.
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Bromodeoxyuridine resistance induced in mouse lymphoma cells by microsomal activation of dimethylnitrosamine.二甲基亚硝胺经微粒体激活后在小鼠淋巴瘤细胞中诱导产生的溴脱氧尿苷抗性
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Bisphenol-A: an estrogenic substance is released from polycarbonate flasks during autoclaving.双酚A:一种雌激素物质在高压灭菌过程中从聚碳酸酯烧瓶中释放出来。
Endocrinology. 1993 Jun;132(6):2279-86. doi: 10.1210/endo.132.6.8504731.
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Gamma irradiation and ethylene oxide in the sterilization of native reindeer bone morphogenetic protein extract.天然驯鹿骨形态发生蛋白提取物灭菌中的伽马射线辐照和环氧乙烷。
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Studies on three mutagen-sensitive mutants of mouse L5178Y cells. I. Characterization of the hybrids between L5178Y and mutagen-sensitive mutants.小鼠L5178Y细胞的三种诱变敏感突变体的研究。I. L5178Y与诱变敏感突变体之间杂种的特性分析。
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J Pharmacobiodyn. 1986 Aug;9(8):688-93. doi: 10.1248/bpb1978.9.688.

本文引用的文献

1
Studies of the culture of leukemic cells in vitro.白血病细胞的体外培养研究。
Ann N Y Acad Sci. 1958 Dec 5;76(3):673-80. doi: 10.1111/j.1749-6632.1958.tb54884.x.
2
A mutational assay system using the thymidine kinase locus in mouse lymphoma cells.一种利用小鼠淋巴瘤细胞中胸苷激酶基因座的突变检测系统。
Mutat Res. 1972 Sep;16(7):77-87. doi: 10.1016/0027-5107(72)90066-8.
3
Laboratory procedure for assessing specific locus mutations at the TK locus in cultured L5178Y mouse lymphoma cells.评估培养的L5178Y小鼠淋巴瘤细胞中胸苷激酶(TK)基因座特定基因座突变的实验室程序。
Mutat Res. 1975 Feb;31(1):17-29. doi: 10.1016/0165-1161(75)90059-x.
4
Toxicity and mutagenicity of radiation from fluorescent lamps and a sunlamp in L5178y mouse lymphoma cells.
Mutat Res. 1978 Jul;51(1):61-75. doi: 10.1016/0027-5107(78)90009-x.