Jockusch B M, Kelley K H, Meyer R K, Burger M M
Histochemistry. 1978 Apr 4;55(3):177-84. doi: 10.1007/BF00495757.
The production of affinity column purified (specific) anti-actin is described. With the immunization scheme employed, all rabbits produced precipitating antibodies over several months, so that 30 mg specific anti-actin per rabbit could be isolated in 6 months. The antibodies against native and detergent denatured smooth muscle actin are characterized by immunodiffusion tests, staining of the I-band of isolated myofibrils and stress fibers in tissue culture cells, using indirect immunofluorescence.
本文描述了亲和柱纯化（特异性）抗肌动蛋白的制备方法。采用所使用的免疫方案，所有兔子在几个月内都产生了沉淀抗体，因此在6个月内每只兔子可分离出30毫克特异性抗肌动蛋白。通过免疫扩散试验、对分离的肌原纤维的I带进行染色以及利用间接免疫荧光对组织培养细胞中的应力纤维进行染色，对针对天然和去污剂变性平滑肌肌动蛋白的抗体进行了表征。
