Nishioka M, Aibiki T, Shirai M, Terada S, Kagawa H, Watanabe S
Microbiol Immunol. 1986;30(12):1291-7. doi: 10.1111/j.1348-0421.1986.tb03061.x.
Actin is a major antigen involved in the reaction of smooth muscle antibody positive sera from patients with chronic active hepatitis. In the present study, actin extracted from rabbit skeletal muscle was denatured by sodium dodecyl sulfate and was immunized into the rabbit, a homologous animal for actin. The rabbits, thus immunized, produced antibodies reactive with actins of homologous and heterologous animals. In addition, the antibodies showed reactivity with autologous actin. It indicates that the denatured homologous actin is capable of terminating immunological tolerance to actin and induces formation of autoantibody to rabbit actin. This phenomenon may be implicated in the occurrence of anti-actin antibody in sera from patients with chronic liver disease and several other diseases.
肌动蛋白是慢性活动性肝炎患者平滑肌抗体阳性血清反应中涉及的主要抗原。在本研究中，从兔骨骼肌中提取的肌动蛋白经十二烷基硫酸钠变性后，免疫注射到兔体内，兔是肌动蛋白的同源动物。经如此免疫的兔子产生了与同源和异源动物的肌动蛋白发生反应的抗体。此外，这些抗体还与自身肌动蛋白发生反应。这表明变性的同源肌动蛋白能够打破对肌动蛋白的免疫耐受，并诱导产生针对兔肌动蛋白的自身抗体。这种现象可能与慢性肝病患者及其他几种疾病患者血清中抗肌动蛋白抗体的产生有关。
