用于区带转头的蔗糖梯度的构建与分析。
The construction and analysis of sucrose gradients for use with zonal rotors.
作者信息
Hirst W, Cox R A
出版信息
Biochem J. 1976 Nov;159(2):259-65. doi: 10.1042/bj1590259.
The rate of sedimentation of a particle in a sucrose solution depends on the viscosity and density of the medium. These two variables are related to the sucrose concentration and the temperature of the medium by new simple equations. These equations were used in a rapid iterative procedure that relates the distance moved by a zone in a continuous sucrose gradient to its sedimentation coefficient. It is shown by comparison with experiment that this iterative method allows the distance moved by a zone to be calculated rapidly. The method may therefore be used to optimize the separation of particles in a sucrose-gradient-centrifugation experiment. The method also allows the unknown sedimentation coefficients of several zones to be measured from a single sucrose-gradient-centrifugation experiment.
蔗糖溶液中颗粒的沉降速率取决于介质的粘度和密度。这两个变量通过新的简单方程与蔗糖浓度和介质温度相关。这些方程被用于一种快速迭代程序，该程序将连续蔗糖梯度中一个区带移动的距离与其沉降系数联系起来。通过与实验比较表明，这种迭代方法能够快速计算区带移动的距离。因此，该方法可用于优化蔗糖梯度离心实验中颗粒的分离。该方法还能从单个蔗糖梯度离心实验中测量几个区带未知的沉降系数。
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