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通过蔗糖梯度超速离心从血清中制备性分离免疫复合物。

Preparative isolation of immune complexes from serum by sucrose gradient ultracentrifugation.

作者信息

Rødahl E, Iversen O J, Dalen A B

出版信息

Scand J Immunol. 1984 Jul;20(1):21-6. doi: 10.1111/j.1365-3083.1984.tb00973.x.

DOI:10.1111/j.1365-3083.1984.tb00973.x
PMID:6463590
Abstract

Immune complexes were isolated by ultracentrifugation in sucrose gradients (20-65% (w/w]. the centrifugation procedure was demonstrated to be isopycnic. The banding density of the complexes was influenced by the chemical nature and molecular size of the antigen and by the antigen to antibody ratio. The method was applied for preparative isolation of immune complexes from patients with systemic lupus erythematosus, rheumatoid arthritis, ankylosing spondylitis, psoriatic arthritis, and uncomplicated psoriasis.

摘要

通过在蔗糖梯度（20 - 65%（w/w））中进行超速离心分离免疫复合物，已证明该离心过程是等密度的。复合物的条带密度受抗原的化学性质和分子大小以及抗原与抗体比例的影响。该方法用于从系统性红斑狼疮、类风湿性关节炎、强直性脊柱炎、银屑病关节炎和单纯性银屑病患者中制备性分离免疫复合物。

相似文献

1
Preparative isolation of immune complexes from serum by sucrose gradient ultracentrifugation.通过蔗糖梯度超速离心从血清中制备性分离免疫复合物。
Scand J Immunol. 1984 Jul;20(1):21-6. doi: 10.1111/j.1365-3083.1984.tb00973.x.
2
Isopycnic ultracentrifugation of immune complexes.免疫复合物的等密度超速离心法。
Scand J Immunol. 1985 Jan;21(1):93-8. doi: 10.1111/j.1365-3083.1985.tb01407.x.
3
Selective losses of large immune complexes during density gradient ultracentrifugation and an approach for prevention of these losses.
J Immunol Methods. 1985 Nov 28;84(1-2):1-10. doi: 10.1016/0022-1759(85)90409-0.
4
Immune complexes in Hodgkin's disease: isolation, immunochemical and physico-chemical analysis.霍奇金病中的免疫复合物：分离、免疫化学及物理化学分析
Clin Exp Immunol. 1983 Aug;53(2):308-18.
5
Analysis of circulating immune complexes from patients with ankylosing spondylitis by gel electrophoresis and immunoblotting using antiserum against a psoriasis associated retrovirus-like particle.通过凝胶电泳和使用抗银屑病相关逆转录病毒样颗粒抗血清的免疫印迹法分析强直性脊柱炎患者的循环免疫复合物。
Ann Rheum Dis. 1986 Nov;45(11):892-8. doi: 10.1136/ard.45.11.892.
6
The production of antibody-dependent cellular cytotoxicity by immune complexes in systemic lupus erythematosus.系统性红斑狼疮中免疫复合物产生抗体依赖性细胞毒性作用。
J Clin Lab Immunol. 1984 Mar;13(3):123-7.
7
A solid-phase assay for circulating immune complexes using monoclonal rheumatoid factors and peroxidase-linked protein A.
J Immunol Methods. 1981;44(1):87-100. doi: 10.1016/0022-1759(81)90110-1.
8
A new gradient former and a simplified procedure for zonal centrifugation of immune complexes.一种用于免疫复合物区带离心的新型梯度形成器及简化程序。
J Immunol Methods. 1979;29(2):173-83. doi: 10.1016/0022-1759(79)90067-x.
9
Isolation of stable aggregates of IgG by zonal ultracentrifugation in sucrose gradients containing albumin.在含有白蛋白的蔗糖梯度中通过区带超速离心法分离IgG稳定聚集体。
Immunol Commun. 1979;8(3):337-45. doi: 10.3109/08820137909050047.
10
The detection of circulating immune complexes containing immunoglobulin G.含有免疫球蛋白G的循环免疫复合物的检测。
J Clin Lab Immunol. 1984 Jun;14(2):73-9.

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The major internal protein, p27, of a retrovirus-like particle participates in immune complex formation in psoriasis.
一种类逆转录病毒颗粒的主要内部蛋白p27参与银屑病中的免疫复合物形成。
Arch Virol. 1985;86(1-2):37-45. doi: 10.1007/BF01314112.
4
Analysis of circulating immune complexes from patients with ankylosing spondylitis by gel electrophoresis and immunoblotting using antiserum against a psoriasis associated retrovirus-like particle.通过凝胶电泳和使用抗银屑病相关逆转录病毒样颗粒抗血清的免疫印迹法分析强直性脊柱炎患者的循环免疫复合物。
Ann Rheum Dis. 1986 Nov;45(11):892-8. doi: 10.1136/ard.45.11.892.
5
Participation of antigens related to the psoriasis associated antigen, pso p27, in immune complex formation in patients with ankylosing spondylitis.与银屑病相关抗原pso p27相关的抗原在强直性脊柱炎患者免疫复合物形成中的作用。
Ann Rheum Dis. 1988 Aug;47(8):628-33. doi: 10.1136/ard.47.8.628.