Leong J K, Schultz W W, Zebovitz E, Schlamm N A
Appl Microbiol. 1973 Sep;26(3):258-63. doi: 10.1128/am.26.3.258-263.1973.
Equipment and techniques previously used to investigate the effect of hyperbaric gases on bacteria were modified to permit comparable investigations with uninfected and virus-infected tissue cell cultures. This report describes the modified equipment and related methodology. Use of the system is illustrated with findings on the effect of oxygen-helium mixtures at 68 atm on cell physiology and virus growth in two cell types. Our results suggested that, under those experimental conditions, several synthetic processes in chick fibroblast monolayers are inhibited but that Sindbis virus growth in the cells is increased. Growth of Japanese encephalitis virus in porcine kidney cells was found to be unaffected by oxygen-helium gas at partial pressures of oxygen between 0 and 700 mm Hg, but morphological alterations in the cells occurred at low and high pO(2) levels.
以前用于研究高压气体对细菌影响的设备和技术经过了改进，以便能够对未感染和病毒感染的组织细胞培养物进行类似的研究。本报告描述了改进后的设备及相关方法。通过在68个大气压下氧氦混合气对两种细胞类型的细胞生理学和病毒生长影响的研究结果，展示了该系统的应用。我们的结果表明，在那些实验条件下，鸡成纤维细胞单层中的几种合成过程受到抑制，但辛德毕斯病毒在细胞中的生长增加。发现在猪肾细胞中，0至700毫米汞柱氧分压下的氧氦气体对日本脑炎病毒的生长没有影响，但在低氧和高氧分压水平下，细胞出现形态学改变。
