Kümel G, Hammer H J
J Gen Virol. 1979 Dec;45(3):599-610. doi: 10.1099/0022-1317-45-3-599.
In culture cells productively infected by adenovirus a high mol. wt. form of DNA is synthesized which is known to represent, at least in part, virus DNA integrated into cellular DNA. We found that the synthesis of this high mol. wt. DNA and the other DNA size classes can strongly and differentially be influenced by altering the metabolic state of the cells. The effects of different rates of cell growth were tested in this respect as well as arginine deprivation as opposed to application of complete growth medium. Synthesis of virus high mol. wt. DNA and unit genome length DNA is enhanced in actively growing as compared to resting Ad5-infected HeLa cells. Under arginine deficiency, in resting Ad5-infected HeLa cells, integration of virus DNA sequences into cellular DNA is almost totally suppressed whereas virus unit genome length DNA is still synthesized. This differential effect is interpreted by the hypothesis that the formation of virus high mol. wt. DNA is a synthetic process that is independent of the unit size virus DNA replication, but coupled to the synthesis of a special form of a special form of cellular DNA that is less effectively shut off by the infection than cellular DNA in general.
在被腺病毒有效感染的培养细胞中，会合成一种高分子量的DNA，已知这种DNA至少部分代表整合到细胞DNA中的病毒DNA。我们发现，通过改变细胞的代谢状态，这种高分子量DNA以及其他DNA大小类别的合成会受到强烈且不同的影响。在这方面，我们测试了不同细胞生长速率的影响，以及与使用完全生长培养基相反的精氨酸剥夺的影响。与静止的Ad5感染的HeLa细胞相比，在活跃生长的细胞中，病毒高分子量DNA和单位基因组长度DNA的合成增强。在精氨酸缺乏的情况下，在静止的Ad5感染的HeLa细胞中，病毒DNA序列整合到细胞DNA中几乎完全受到抑制，而病毒单位基因组长度DNA仍在合成。这种差异效应可以通过以下假设来解释：病毒高分子量DNA的形成是一个合成过程，它独立于单位大小的病毒DNA复制，但与一种特殊形式的细胞DNA的合成相关，这种细胞DNA在感染时比一般的细胞DNA更不容易被关闭。
