采用气液色谱-质谱联用技术鉴定大鼠尿液中的黄烷酮代谢产物。
Identification of flavanone metabolites in rat urine by combined gas-liquid chromatography and mass spectrometry.
作者信息
Das N P, Scott K N, Duncan J H
出版信息
Biochem J. 1973 Dec;136(4):903-9. doi: 10.1042/bj1360903.
Abstract
- The metabolism of flavanone in the rat was studied after oral or intraperitoneal administration of the compound. Flavone and flav-3-ene together with five other unidentified minor metabolites were excreted in the urine. 2. The formation of flavanone metabolites was not suppressed by the administration of high doses of the antibacterial compounds aureomycin and phthaloylsulphathiazole. 3. No aromatic acids that could be attributed to ring cleavage of flavanone were detected. 4. Administration of 100 or 200mg of flavanone daily per rat caused some deaths during the 7-14-day period. 5. The application of combined gas-liquid chromatography/mass spectrometry and proton nuclear-magnetic-resonance spectroscopy to the separation and identification of the flavanone metabolites is described. 6. Measurement of the two major flavanone metabolites was carried out by gas-liquid chromatography.
摘要
- 在大鼠经口或腹腔注射该化合物后，对黄烷酮的代谢情况进行了研究。黄酮和黄烷 -3- 烯以及其他五种未鉴定的次要代谢产物随尿液排出。2. 高剂量抗菌化合物金霉素和酞磺胺噻唑的给药并未抑制黄烷酮代谢产物的形成。3. 未检测到可归因于黄烷酮环裂解的芳香酸。4. 每只大鼠每日给予100或200毫克黄烷酮，在7至14天期间导致了一些死亡。5. 描述了应用气液色谱/质谱联用和质子核磁共振光谱法对黄烷酮代谢产物进行分离和鉴定的过程。6. 通过气液色谱法对两种主要的黄烷酮代谢产物进行了测定。
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