Gaben-Cogneville A M, Jahchan T, Swierczewski E
J Nutr. 1981 Dec;111(12):2098-105. doi: 10.1093/jn/111.12.2098.
The effect of litter size on the incorporation of labeled thymidine (TdR) into DNA was studied in the stromal and the adipocyte fractions of the rat inguinal tissue. In experiment 1 the animal were kept in litters of 18 (UF) or 6 (control) from birth till 10 days. They were injected with [2-14C] TdR at day 3 and killed at 60 minutes, 1, 3 and 7 days post-injection. In experiment 2, the pups were raised in litters of 18 during 3 (RF3), 6 (RF6) or 10 (RF10) days, and distributed again in litters of six. They were injected with [2-14C] TdR or [14CH3]TdR at the beginning of the refeeding and killed as described previously. In all experiments the weight of the inguinal tissue was more reduced than the total body weight. In the UF, the proliferation was markedly reduced in cellular fractions as was the differentiation of stromal cells into adipocytes from six days of underfeeding. In the RF3 and the RF6 there was an attempt to recover the cell number as suggested by the recycling of the degradation products of TdR for DNA synthesis. In the RF10, cell multiplication and differentiation were strongly affected by the length of the deprivation period.
研究了窝仔数对标记胸腺嘧啶核苷（TdR）掺入大鼠腹股沟组织基质和脂肪细胞部分DNA的影响。在实验1中，动物从出生到10天分别饲养在18只（超量饲养组，UF）或6只（对照组）的窝中。在第3天给它们注射[2-¹⁴C]TdR，并在注射后60分钟、1天、3天和7天处死。在实验2中，幼崽在18只的窝中饲养3天（再喂养3天组，RF3）、6天（再喂养6天组，RF6）或10天（再喂养10天组，RF10），然后再分成6只的窝。在重新喂养开始时给它们注射[2-¹⁴C]TdR或[¹⁴CH₃]TdR，并按先前描述的方法处死。在所有实验中，腹股沟组织的重量比总体重减少得更多。在超量饲养组中，从限食6天起，细胞部分的增殖明显减少，基质细胞向脂肪细胞的分化也减少。在再喂养3天组和再喂养6天组中，正如TdR降解产物再循环用于DNA合成所表明的那样，有细胞数量恢复的趋势。在再喂养10天组中，细胞增殖和分化受到剥夺期长度的强烈影响。
