小牛胸腺DNA聚合酶α部分纯化的进一步研究
Further studies on partially purified calf thymus DNA polymerase a.
作者信息
McKune K, Holmes A M
出版信息
Nucleic Acids Res. 1979 Jul 25;6(10):3341-52. doi: 10.1093/nar/6.10.3341.
Abstract
Attempts to prevent the urea conversion of a 200-230,000 molecular weight DNA polymerase alpha to a 150-170,000 molecular weight form by the inclusion of protease inhibitors have not been successful. No other method has been found capable of dissociating a 50-70,000 fragment or subunit from the DNA polymerase subunit. Addition of this 50-70,000 subunit to the polymerase subunit does not aid the binding of the enzyme to DNA, but does have an effect on the utilisation of synthetic template-initiator complexes by the polymerase subunit.
摘要
通过加入蛋白酶抑制剂来阻止分子量为200000 - 230000的DNA聚合酶α转化为分子量为150000 - 170000的形式的尝试未获成功。尚未发现其他方法能够从DNA聚合酶亚基上解离出一个50000 - 70000的片段或亚基。将这个50000 - 70000的亚基添加到聚合酶亚基上，对该酶与DNA的结合并无帮助，但确实会对聚合酶亚基利用合成模板 - 引发剂复合物产生影响。
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