游离核糖体组分被先前附着于膜上的核糖体所污染。
Contamination of free-ribosome fractions by detached previously membrane-bound ribosomes.
作者信息
O'Toole K
出版信息
Biochem J. 1974 Feb;138(2):305-7. doi: 10.1042/bj1380305.
Abstract
A rough-membrane fraction isolated from rat liver by a procedure designed to prevent membrane denaturation was subjected to the gradient treatment normally used to isolate free ribosomes. Under these conditions, at most 20% of the ribosomes were detached from membrane with less than 5% sedimenting into the free-polyribosome pellet.
摘要
通过一种旨在防止膜变性的方法从大鼠肝脏中分离出的粗膜部分，接受了通常用于分离游离核糖体的梯度处理。在这些条件下，最多20%的核糖体从膜上脱离，不到5%沉淀到游离多核糖体沉淀中。
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