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游离核糖体组分被先前附着于膜上的核糖体所污染。

Contamination of free-ribosome fractions by detached previously membrane-bound ribosomes.

作者信息

O'Toole K

出版信息

Biochem J. 1974 Feb;138(2):305-7. doi: 10.1042/bj1380305.

DOI:10.1042/bj1380305
PMID:4822735
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1166208/
Abstract

A rough-membrane fraction isolated from rat liver by a procedure designed to prevent membrane denaturation was subjected to the gradient treatment normally used to isolate free ribosomes. Under these conditions, at most 20% of the ribosomes were detached from membrane with less than 5% sedimenting into the free-polyribosome pellet.

摘要

通过一种旨在防止膜变性的方法从大鼠肝脏中分离出的粗膜部分,接受了通常用于分离游离核糖体的梯度处理。在这些条件下,最多20%的核糖体从膜上脱离,不到5%沉淀到游离多核糖体沉淀中。

相似文献

1
Contamination of free-ribosome fractions by detached previously membrane-bound ribosomes.游离核糖体组分被先前附着于膜上的核糖体所污染。
Biochem J. 1974 Feb;138(2):305-7. doi: 10.1042/bj1380305.
2
Studies on free and membrane-bound ribosomes in rat liver. I. Distribution as related to total cellular RNA.大鼠肝脏中游离核糖体和附着核糖体的研究。I. 与细胞总RNA相关的分布情况
J Mol Biol. 1967 Jun 14;26(2):279-92. doi: 10.1016/0022-2836(67)90297-5.
3
Membrane-bound ribosomes of myeloma cells. I. Preparation of free and membrane-bound ribosomal fractions. Assessment of the methods and properties of the ribosomes.骨髓瘤细胞膜结合核糖体。I. 游离及膜结合核糖体组分的制备。核糖体方法及性质评估。
J Cell Biol. 1975 Oct;67(1):1-15. doi: 10.1083/jcb.67.1.1.
4
A rapidly sedimenting fraction of rat liver endoplasmic reticulum.
J Cell Sci. 1973 Sep;13(2):447-59. doi: 10.1242/jcs.13.2.447.
5
Aggregation properties of rat liver ribosomes.大鼠肝脏核糖体的聚集特性。
Basic Life Sci. 1973;1:267-76. doi: 10.1007/978-1-4684-0877-5_22.
6
Free and membrane-bound ribosomes in rat liver.大鼠肝脏中的游离核糖体和附着核糖体。
J Biol Chem. 1967 May 10;242(9):2069-74.
7
Physicochemical characteristics of isolated 55-S mitochondrial ribosomes from rat-liver.从大鼠肝脏中分离出的55-S线粒体核糖体的物理化学特性
Biochem Biophys Res Commun. 1973 Sep 5;54(1):308-14. doi: 10.1016/0006-291x(73)90924-8.
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Distribution of free and membrane-bound ribosomes in livers of protein-calorie-deficient rats.蛋白质 - 热量缺乏型大鼠肝脏中游离核糖体和膜结合核糖体的分布
Lab Invest. 1972 Jun;26(6):626-36.
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An improved cell fractionation procedure for the preparation of rat liver membrane-bound ribosomes.一种用于制备大鼠肝脏膜结合核糖体的改进细胞分级分离程序。
J Cell Biol. 1973 Jan;56(1):191-205. doi: 10.1083/jcb.56.1.191.
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Isopycnic centrifugation of free and membrane-bound polysomes from rat liver.大鼠肝脏游离及膜结合多核糖体的等密度离心。
Eur J Biochem. 1974 May 2;44(1):161-70. doi: 10.1111/j.1432-1033.1974.tb03469.x.

引用本文的文献

1
Polyribosomes from Peas: V. An Attempt to Characterize the Total Free and Membrane-bound Polysomal Population.豌豆多核糖体:V. 尝试对总游离和膜结合多核糖体群体进行特征分析。
Plant Physiol. 1975 Apr;55(4):749-56. doi: 10.1104/pp.55.4.749.
2
The binding of monoribosomes, oligoribosomes and polyribosomes to reticular membranes from rat liver.单核糖体、寡核糖体和多核糖体与大鼠肝脏网状膜的结合。
Biochem J. 1978 Apr 15;172(1):109-14. doi: 10.1042/bj1720109.

本文引用的文献

1
A small particulate component of the cytoplasm.细胞质的一种小颗粒成分。
J Biophys Biochem Cytol. 1955 Jan;1(1):59-68. doi: 10.1083/jcb.1.1.59.
2
PREPARATION OF RAT-LIVER POLYSOMES WITHOUT THE UTILIZATION OF DETERGENT.不使用去污剂制备大鼠肝脏多核糖体
Biochim Biophys Acta. 1964 May 18;87:177-80. doi: 10.1016/0926-6550(64)90064-7.
3
Studies on free and membrane-bound ribosomes in rat liver. I. Distribution as related to total cellular RNA.大鼠肝脏中游离核糖体和附着核糖体的研究。I. 与细胞总RNA相关的分布情况
J Mol Biol. 1967 Jun 14;26(2):279-92. doi: 10.1016/0022-2836(67)90297-5.
4
Isolation and properties of polyribosomes and fragments of the endoplasmic reticulum from rat liver.大鼠肝脏多核糖体和内质网片段的分离及特性
Biochem J. 1967 Apr;103(1):177-82. doi: 10.1042/bj1030177.
5
Free and membrane-bound ribosomes in rat liver.大鼠肝脏中的游离核糖体和附着核糖体。
J Biol Chem. 1967 May 10;242(9):2069-74.
6
Electron microscopic observation on polyribosomes and endoplasmic reticulum fragments isolated from rat liver.
Lab Invest. 1966 Jan;15(1 Pt 1):196-208.
7
Biosynthesis of serum proteins and ferritin by free and attached ribosomes of rat liver.大鼠肝脏游离核糖体和附着核糖体对血清蛋白及铁蛋白的生物合成
J Biol Chem. 1969 Aug 25;244(16):4308-15.
8
Recent developments in the measurement of nucleic acids in biological materials. A supplementary review.生物材料中核酸测量的最新进展。补充综述。
Analyst. 1966 Feb;91(79):78-88. doi: 10.1039/an9669100078.
9
Ribosome monomers in rat liver following administration of dimethylnitrosamine.给予二甲基亚硝胺后大鼠肝脏中的核糖体单体。
Cancer Res. 1971 Dec;31(12):2189-95.
10
Protein synthesis by membrane-bound and free ribosomes of secretory and non-secretory tissues.分泌性和非分泌性组织的膜结合核糖体和游离核糖体的蛋白质合成。
Biochem J. 1971 Feb;121(4):683-94. doi: 10.1042/bj1210683.