Fraser D, Fleischmann C
J Virol. 1974 May;13(5):1067-74. doi: 10.1128/JVI.13.5.1067-1074.1974.
We have studied the adsorption of the Gourlay Acholeplasma virus MVL-1 to the host cell Acholeplasma laidlawii JA-1. Successful adsorption depends primarily on some unknown action of the serum factor in the medium, but evaluation of various physical parameters indicates clearly that given this factor the kinetics is pseudo first order (K = 3 x 10(-9) cm(3)/min) and the mechanism ionic. Chiefly important are the ionic strength of the cation and pH (optimal Na(+) = 0.08 M, pH = 6). The system seems indifferent to whether the cation is Na, K, NH(4), Ca, or Mg. There is little effect of temperature over the range 0 to 42 C. The diffusion constant of the virus, calculated from its geometry or its maximum adsorption rate, is consistent with its reported size and shape.
我们研究了古莱无胆甾原体病毒MVL-1对宿主细胞莱氏无胆甾原体JA-1的吸附作用。成功吸附主要取决于培养基中血清因子的某些未知作用，但对各种物理参数的评估清楚地表明，在有该因子的情况下，动力学为假一级反应（K = 3×10⁻⁹ cm³/分钟），且机制为离子性。主要重要的是阳离子的离子强度和pH值（最佳Na⁺ = 0.08 M，pH = 6）。该系统似乎对阳离子是Na、K、NH₄、Ca还是Mg并不在意。在0至42℃范围内，温度影响很小。根据病毒的几何形状或其最大吸附速率计算出的病毒扩散常数，与报道的其大小和形状一致。
