Rottem S, Greenberg N
Yale J Biol Med. 1983 Sep-Dec;56(5-6):765-9.
Binding of MVL-2 virus, whose envelope lipids were radioactively labeled, to A. laidlawii JA1 cells was determined and characterized. The binding followed first-order kinetics and was temperature-dependent. All MVL-2 particles were capable of binding to A. laidlawii cells. At least 75 percent of radioactive MVL-2 bound represented specific binding which was markedly inhibited by EDTA. Virus infectivity was not essential for binding as inactivation of the virus by ultraviolet irradiation did not affect binding. Nevertheless, protein denaturing agents or proteolytic enzymes markedly inhibited MVL-2 binding, suggesting that the binding site of MVL-2 is of proteinaceous nature.
对包膜脂质经放射性标记的MVL - 2病毒与莱氏无胆甾原体JA1细胞的结合进行了测定和表征。这种结合遵循一级动力学，且依赖于温度。所有MVL - 2颗粒都能够与莱氏无胆甾原体细胞结合。结合的放射性MVL - 2中至少75%代表特异性结合，而EDTA可显著抑制这种特异性结合。病毒感染性对于结合并非必需，因为紫外线照射使病毒失活并不影响结合。然而，蛋白质变性剂或蛋白水解酶可显著抑制MVL - 2的结合，这表明MVL - 2的结合位点具有蛋白质性质。
