有尾支原体病毒L3对细胞膜的吸附。
Adsorption of the tailed mycoplasma virus L3 to cell membranes.
作者信息
Haberer K, Maniloff J
出版信息
J Virol. 1982 Feb;41(2):501-7. doi: 10.1128/JVI.41.2.501-507.1982.
Abstract
The adsorption properties of the tailed bacteriophage L3 to Acholeplasma laidlawii cells were studied. Adsorption followed a biphasic curve. Reversibility and virus heterogeneity were not sufficient to explain the break in the adsorption curve. Binding studies showed that each colony-forming unit could bind about 350 virions. The electrostatic nature of L3 adsorption was indicated by the effect of cations, pH, and temperature on the adsorption rate constant. L3 adsorption appeared to have a requirement for Ca2+, which could not be replaced by the mono- and divalent cations examined. Ethylene glycol-bis(beta-aminoethyl ether)-N,N-tetraacetic acid inhibition of adsorption was totally reversed by added Ca2+. The effects of EDTA, proteases, and lectins on absorption indicated that membrane proteins are the L3 receptors. The model for L3 adsorption is a multivalent one involving lateral diffusion of adsorbed virions and receptor proteins.
摘要
研究了尾噬菌体L3对莱氏无胆甾原体细胞的吸附特性。吸附呈双相曲线。吸附曲线的转折点不能用可逆性和病毒异质性来充分解释。结合研究表明，每个集落形成单位可结合约350个病毒粒子。阳离子、pH值和温度对吸附速率常数的影响表明L3吸附具有静电性质。L3吸附似乎需要Ca2+，所检测的单价和二价阳离子均不能替代它。添加Ca2+可完全逆转乙二醇双（β-氨基乙醚）-N，N-四乙酸对吸附的抑制作用。EDTA、蛋白酶和凝集素对吸附的影响表明膜蛋白是L3的受体。L3吸附模型是一个多价模型，涉及吸附病毒粒子和受体蛋白的侧向扩散。
相似文献
1
Adsorption of the tailed mycoplasma virus L3 to cell membranes.有尾支原体病毒L3对细胞膜的吸附。
J Virol. 1982 Feb;41(2):501-7. doi: 10.1128/JVI.41.2.501-507.1982.
2
Active diffusion of adsorbed mycoplasmal virus L3 on Acholeplasma laidlawii cell membranes.
Rev Infect Dis. 1982 May-Jun;4 Suppl:S105-8. doi: 10.1093/clinids/4.supplement_1.s105.
3
Virus capping on mycoplasma cells and its effect on membrane structure.
Biochim Biophys Acta. 1982 Jun 28;688(3):720-6. doi: 10.1016/0005-2736(82)90284-x.
4
Receptor sites for mycoplasmal viruses on Acholeplasma laidlawii.莱氏无胆甾原体上支原体病毒的受体位点。
Rev Infect Dis. 1982 May-Jun;4 Suppl:S109-14. doi: 10.1093/clinids/4.supplement_1.s109.
5
Lateral mobility of membrane-bound antibodies on the surface of Acholeplasma laidlawii: evidence for virus-induced cell fusion in a procaryote.莱氏无胆甾原体表面膜结合抗体的侧向移动性：原核生物中病毒诱导细胞融合的证据。
J Bacteriol. 1982 Oct;152(1):471-8. doi: 10.1128/jb.152.1.471-478.1982.
6
Effects of acyl chain composition on production of mycoplasmavirus MV-L2 by Acholeplasma laidlawii.
Isr J Med Sci. 1984 Sep;20(9):788-92.
7
Adsorption, capping, and release of a complex bacteriophage by mycoplasma cells.
J Virol. 1980 Oct;36(1):264-70. doi: 10.1128/JVI.36.1.264-270.1980.
8
Infection of Acholeplasma laidlawii by MVL51 virus.莱氏无胆甾原体被MVL51病毒感染。
Virology. 1973 Sep;55(1):118-26. doi: 10.1016/s0042-6822(73)81013-x.
9
Interaction of mycoplasma with viruses. I. Primary adsorption of virus is ionic in mechanism.支原体与病毒的相互作用。I. 病毒的初次吸附在机制上是离子性的。
J Virol. 1974 May;13(5):1067-74. doi: 10.1128/JVI.13.5.1067-1074.1974.
10
Electroporation-mediated transfection of Acholeplasma laidlawii with mycoplasma virus L1 and L3 DNA.用电穿孔法将支原体病毒L1和L3 DNA转染莱氏无胆甾原体。
J Virol. 1988 Aug;62(8):3050-2. doi: 10.1128/JVI.62.8.3050-3052.1988.
引用本文的文献
1
Identification and molecular characterization of bacteriophage phiAxp-2 of Achromobacter xylosoxidans.木糖氧化无色杆菌噬菌体phiAxp-2的鉴定及分子特征分析
Sci Rep. 2016 Sep 27;6:34300. doi: 10.1038/srep34300.
2
Isolation and molecular characterisation of Achromobacter phage phiAxp-3, an N4-like bacteriophage.无色杆菌噬菌体phiAxp-3（一种N4样噬菌体）的分离与分子特征分析
Sci Rep. 2016 Apr 20;6:24776. doi: 10.1038/srep24776.
3
Characterization of a novel Achromobacter xylosoxidans specific siphoviruse: phiAxp-1.一种新型木糖氧化无色杆菌特异性肌尾噬菌体的特性研究：phiAxp-1
Sci Rep. 2016 Feb 24;6:21943. doi: 10.1038/srep21943.
4
Evidence that the heterogeneity of a T4 population is the result of heritable traits.T4群体的异质性是可遗传性状的结果的证据。
PLoS One. 2014 Dec 31;9(12):e116235. doi: 10.1371/journal.pone.0116235. eCollection 2014.
5
Synergistic action of gentamicin and bacteriophage in a continuous culture population of Staphylococcus aureus.庆大霉素和噬菌体在金黄色葡萄球菌连续培养群体中的协同作用。
PLoS One. 2012;7(11):e51017. doi: 10.1371/journal.pone.0051017. Epub 2012 Nov 30.
6
Lateral mobility of membrane-bound antibodies on the surface of Acholeplasma laidlawii: evidence for virus-induced cell fusion in a procaryote.莱氏无胆甾原体表面膜结合抗体的侧向移动性：原核生物中病毒诱导细胞融合的证据。
J Bacteriol. 1982 Oct;152(1):471-8. doi: 10.1128/jb.152.1.471-478.1982.
7
Characterization of the enveloped plasmavirus MVL2 after propagation on three Acholeplasma laidlawii hosts.
Arch Virol. 1983;75(1-2):123-9. doi: 10.1007/BF01314132.
8
Transfection of REP- mycoplasmas with viral single-stranded DNA.用病毒单链DNA转染REP支原体。
J Virol. 1985 Jan;53(1):25-31. doi: 10.1128/JVI.53.1.25-31.1985.
9
Molecular biology and genetics of mycoplasmas (Mollicutes).支原体（柔膜菌纲）的分子生物学与遗传学
Microbiol Rev. 1985 Dec;49(4):419-55. doi: 10.1128/mr.49.4.419-455.1985.
10
Adsorption of mycoplasmavirus MV-L2 to Acholeplasma laidlawii: effects of changes in the acyl-chain composition of membrane lipids.支原体病毒MV-L2对莱氏无胆甾原体的吸附：膜脂酰基链组成变化的影响
J Virol. 1986 Nov;60(2):525-30. doi: 10.1128/JVI.60.2.525-530.1986.
本文引用的文献
1
The first two steps of the invasion of host cells by bacterial viruses. II.细菌病毒入侵宿主细胞的前两个步骤。II.
J Exp Med. 1951 Sep;94(3):177-89. doi: 10.1084/jem.94.3.177.
2
Attachment and penetration of cells by viruses.病毒对细胞的附着与穿透。
Adv Virus Res. 1957;4:63-110. doi: 10.1016/s0065-3527(08)60596-5.
3
Adsorption, capping, and release of a complex bacteriophage by mycoplasma cells.
J Virol. 1980 Oct;36(1):264-70. doi: 10.1128/JVI.36.1.264-270.1980.
4
Phylogenetic analysis of the mycoplasmas.支原体的系统发育分析。
Proc Natl Acad Sci U S A. 1980 Jan;77(1):494-8. doi: 10.1073/pnas.77.1.494.
5
The kinetics of reversible and irreversible attachment of bacteriophage T-1.噬菌体T-1可逆和不可逆附着的动力学
Virology. 1965 Aug;26(4):727-37. doi: 10.1016/0042-6822(65)90336-3.
6
Divalent cations in native and reaggregated mycoplasma membranes.天然及重聚集支原体膜中的二价阳离子
J Bacteriol. 1973 Feb;113(2):666-71. doi: 10.1128/jb.113.2.666-671.1973.
7
Characterization of the mycoplasma membrane proteins. II. Solubilization and enzymic activities of Acholeplasma laidlawii membrane proteins.
Biochim Biophys Acta. 1971 Oct 12;249(1):169-76. doi: 10.1016/0005-2736(71)90093-9.
8
Studies with lectins on the surface carbohydrate structures of mycoplasma membranes.关于凝集素对支原体细胞膜表面碳水化合物结构的研究。
J Bacteriol. 1974 Oct;120(1):81-8. doi: 10.1128/jb.120.1.81-88.1974.
9
Binding of plant lectins to mycoplasma cells and membranes.植物凝集素与支原体细胞及细胞膜的结合。
J Bacteriol. 1976 Oct;128(1):1-7. doi: 10.1128/jb.128.1.1-7.1976.
10
Structural and biological properties of mycoplasmavirus MVL3: an unusual virus-procaryote interaction.支原体病毒MVL3的结构与生物学特性：一种不同寻常的病毒-原核生物相互作用
J Virol. 1979 Oct;32(1):268-75. doi: 10.1128/JVI.32.1.268-275.1979.
Zotero 插件