Haberer K, Maniloff J
J Virol. 1982 Feb;41(2):501-7. doi: 10.1128/JVI.41.2.501-507.1982.
The adsorption properties of the tailed bacteriophage L3 to Acholeplasma laidlawii cells were studied. Adsorption followed a biphasic curve. Reversibility and virus heterogeneity were not sufficient to explain the break in the adsorption curve. Binding studies showed that each colony-forming unit could bind about 350 virions. The electrostatic nature of L3 adsorption was indicated by the effect of cations, pH, and temperature on the adsorption rate constant. L3 adsorption appeared to have a requirement for Ca2+, which could not be replaced by the mono- and divalent cations examined. Ethylene glycol-bis(beta-aminoethyl ether)-N,N-tetraacetic acid inhibition of adsorption was totally reversed by added Ca2+. The effects of EDTA, proteases, and lectins on absorption indicated that membrane proteins are the L3 receptors. The model for L3 adsorption is a multivalent one involving lateral diffusion of adsorbed virions and receptor proteins.
研究了尾噬菌体L3对莱氏无胆甾原体细胞的吸附特性。吸附呈双相曲线。吸附曲线的转折点不能用可逆性和病毒异质性来充分解释。结合研究表明，每个集落形成单位可结合约350个病毒粒子。阳离子、pH值和温度对吸附速率常数的影响表明L3吸附具有静电性质。L3吸附似乎需要Ca2+，所检测的单价和二价阳离子均不能替代它。添加Ca2+可完全逆转乙二醇双（β-氨基乙醚）-N，N-四乙酸对吸附的抑制作用。EDTA、蛋白酶和凝集素对吸附的影响表明膜蛋白是L3的受体。L3吸附模型是一个多价模型，涉及吸附病毒粒子和受体蛋白的侧向扩散。
