Musher D M, Griffith D P, Tyler M, Woelfel A
Antimicrob Agents Chemother. 1974 Feb;5(2):101-5. doi: 10.1128/AAC.5.2.101.
In vitro testing shows nearly all strains of Proteus to be susceptible to methenamine. However, infection by urease-producing bacteria alkalinizes the urine in vivo and prevents generation of formaldehyde, the active metabolite, from methenamine. We have previously shown acetohydroxamic acid (AHA) to be an effective inhibitor of bacterial urease in vitro and in vivo. We now present data obtained by use of static and dynamic in vitro systems, which show that, by preventing urease-induced alkalinization of urine, AHA enables methenamine to exert its antibacterial effect against representative Proteus species.
体外试验表明，几乎所有变形杆菌菌株对乌洛托品敏感。然而，产脲酶细菌感染在体内会使尿液碱化，并阻止乌洛托品生成活性代谢产物甲醛。我们之前已表明，乙酰氧肟酸（AHA）在体外和体内都是一种有效的细菌脲酶抑制剂。我们现在展示通过使用静态和动态体外系统获得的数据，这些数据表明，通过防止脲酶诱导的尿液碱化，AHA能使乌洛托品对代表性变形杆菌发挥抗菌作用。
