Hammerström J, Unsgaard G, Lamvik J
Acta Pathol Microbiol Scand C. 1979 Jun;87C(3):167-75.
Human monocytes activated in vitro by lymphokine-containing supernatants of autologous or allogeneic lymphocytes stimulated in vitro by Corynebacterium parvum (CP) expressed increased ability to suppress DNA-synthesis in a human tumour cell line. Monocyte activation was not dependent on in vitro differentiation of monocytes, enhanced cytostatic ability being observed at all stages of in vitro differentiation. The lymphokine-induced cytostatic ability was not affected by intensive washing and trypsin treatment of the activated monocytes, but disappeared during 48 hours of in vitro culture of the activated cells. The increased cytostatic ability of lymphokine-activated monocytes did not seem to be due to stable supernatant factors released from monocytes. CP stimulated DNA-synthesis in peripheral blood lymphocytes of 28 normal donors, thus confirming the mitogenic effect of CP on human lymphocytes. Lymphokine production in response to CP correlated with the magnitude of DNA-synthesis, but appeared before DNA-synthesis could be detected in the lymphocytes.
用微小棒状杆菌（CP）体外刺激自体或同种异体淋巴细胞产生的含淋巴因子的上清液在体外激活人单核细胞，结果显示这些单核细胞抑制人肿瘤细胞系中DNA合成的能力增强。单核细胞的激活并不依赖于单核细胞的体外分化，在体外分化的各个阶段均观察到其抑制细胞生长能力增强。淋巴因子诱导的抑制细胞生长能力不受对激活的单核细胞进行充分洗涤和胰蛋白酶处理的影响，但在激活细胞的体外培养48小时期间消失。淋巴因子激活的单核细胞抑制细胞生长能力的增强似乎并非由于单核细胞释放的稳定上清液因子所致。CP刺激了28名正常供体外周血淋巴细胞中的DNA合成，从而证实了CP对人淋巴细胞的促有丝分裂作用。对CP产生的淋巴因子与DNA合成的程度相关，但在淋巴细胞中检测到DNA合成之前就已出现。
