Bieri J G, Tolliver T J, Catignani G L
Am J Clin Nutr. 1979 Oct;32(10):2143-9. doi: 10.1093/ajcn/32.10.2143.
This paper describes a rapid, microprocedure for the simultaneous determination of alpha-tocopherol (vitamin E) and retinol (vitamin A) in plasma, and of alpha-tocopherol alone in red cells since cells do not contain retinol. A total lipid extract from 0.1 ml plasma or 0.125 ml red cells and containing internal standards of alpha-tocopheryl acetate and retinyl acetates is injected onto a high pressure liquid chromatography with a reverse phase column developed with methanol-water. An ultraviolet detector with 280-nm filter is used. The chromatogram is complete in 8 min and the alpha-tocopherol and retinol are quantitated by the peak height ratio method. Comparison of results with both plasma and red cells gave excellent agreement with conventional methods for these vitamins. The procedure should be particularly useful for clinical studies and nutrition surveys.
本文描述了一种快速的微量方法，用于同时测定血浆中的α-生育酚（维生素E）和视黄醇（维生素A），以及红细胞中单独的α-生育酚，因为细胞不含视黄醇。将来自0.1 ml血浆或0.125 ml红细胞的总脂质提取物（含有α-生育酚醋酸酯和视黄醇醋酸酯内标）注入到用甲醇-水展开的反相柱高压液相色谱仪中。使用带有280 nm滤光片的紫外检测器。8分钟内完成色谱图，通过峰高比法对α-生育酚和视黄醇进行定量。血浆和红细胞结果的比较与这些维生素的传统方法非常吻合。该方法对临床研究和营养调查应特别有用。
