[Methodological approaches to developing a method of regulated antibiotic biosynthesis].

Methodologic approaches to the development of methods for controlled biosynthesis of antibiotics are discussed with reference to oxytetracycline biosynthesis. The method of acute experiments in conjunction with mathematical methods of experiment planning was used for determination of the substrate concentrations optimal for the culture growth and biosynthesis of oxytetracycline. It was found that such mathematic procedure provided simultaneous determination of optimal concentrations of all the substrates.