使用纯化的卡那霉素乙酰转移酶对阿米卡星进行分光光度测定。
Spectrophotometric assay for amikacin using purified kanamycin acetyltransferase.
作者信息
Scarbrough E, Williams J W, Northrop D B
出版信息
Antimicrob Agents Chemother. 1979 Aug;16(2):221-4. doi: 10.1128/AAC.16.2.221.
Abstract
A rapid spectrophotometric assay has been developed for measuring the concentrations of amikacin and related antibiotics in serum. The assay uses a purified enzyme from R-factor E. coli which acetylates amikacin with the production of coenzyme A, the latter in turn being reacted with a sulfhydryl reagent to produce stoichiometric amounts of a sensitive chromophore, that is measured in the visible spectrum. The system complements an earlier assay for gentamicin-related antibiotics thereby facilitating the rapid measurement of the concentrations of all clinically important aminoglycosides in serum.
摘要
已开发出一种快速分光光度测定法，用于测量血清中阿米卡星及相关抗生素的浓度。该测定法使用来自R因子大肠杆菌的纯化酶，该酶使阿米卡星乙酰化并产生辅酶A，后者再与巯基试剂反应，产生化学计量的灵敏发色团，在可见光谱中进行测量。该系统补充了早期针对庆大霉素相关抗生素的测定法，从而有助于快速测量血清中所有临床上重要的氨基糖苷类药物的浓度。
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本文引用的文献
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Antimicrob Agents Chemother. 1974 Sep;6(3):316-9. doi: 10.1128/AAC.6.3.316.
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Tissue sulfhydryl groups.组织巯基
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Enzymatic acetylation of aminoglycoside antibiotics by Escherichia coli carrying an R factor.携带R因子的大肠杆菌对氨基糖苷类抗生素的酶促乙酰化作用。
Biochemistry. 1971 May 11;10(10):1787-96. doi: 10.1021/bi00786a009.
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Amikacin: a rapid and sensitive radioimmunoassay.阿米卡星：一种快速灵敏的放射免疫测定法。
Antimicrob Agents Chemother. 1975 Jan;7(1):42-5. doi: 10.1128/AAC.7.1.42.
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7
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