Rosan B, Hammond B F
Infect Immun. 1974 Aug;10(2):304-8. doi: 10.1128/iai.10.2.304-308.1974.
Polysaccharide(s) have been isolated from supernatants of broth cultures of Actinomyces viscosus, strain T6. The organism produces significant quantities (2 mg/mg of cell [dry weight], 108 mg/liter) of this polysaccharide in the absence of sucrose. Chemical analysis indicated that N-acetylglucosamine (62%) was the major component; galactose (7%), glucose (4%), uronic acid (3%), and smaller amounts of glycerol, rhamnose, arabinose, and xylose were also present. The polysaccharide(s) is only slightly soluble in aqueous solutions. Although further purification of the polysaccharides will be necessary before definitive chemical and structural studies, the formation of extracellular polysaccharides is significant because it offers a possible explanation for plaque formation by these organisms.
已从粘性放线菌T6菌株肉汤培养物的上清液中分离出多糖。该微生物在没有蔗糖的情况下能产生大量(每毫克细胞[干重]2毫克,每升108毫克)这种多糖。化学分析表明,N - 乙酰葡糖胺(62%)是主要成分;还存在半乳糖(7%)、葡萄糖(4%)、糖醛酸(3%)以及少量的甘油、鼠李糖、阿拉伯糖和木糖。该多糖在水溶液中仅微溶。尽管在进行明确的化学和结构研究之前有必要对多糖进行进一步纯化,但细胞外多糖的形成具有重要意义,因为它为这些微生物形成菌斑提供了一种可能的解释。