Cooper H R, Chorpenning F W, Rosen S
Infect Immun. 1975 Apr;11(4):823-8. doi: 10.1128/iai.11.4.823-828.1975.
Purified cell walls from Streptococcus mutans strain BHT were prepared without the use of proteolytic enzymes in order to retain all cell wall constituents for chemical analysis. Of four methods employed, the Ribi cell fractionator produced disrupted cell suspensions which could be most thoroughly purified on sucrose gradients. Results of chemical analyses on purified cell walls prepared in this 8.9% glycerol teichoic acid, 33.6% non-peptidoglycan polysaccharide, and 49.9% peptidoglycan.
为保留所有细胞壁成分用于化学分析，采用不使用蛋白水解酶的方法制备了变形链球菌BHT菌株的纯化细胞壁。在所采用的四种方法中，Ribi细胞分级分离仪产生的破碎细胞悬液在蔗糖梯度上能得到最彻底的纯化。对用这种方法制备的纯化细胞壁进行化学分析的结果表明，其含有8.9%的甘油磷壁酸、33.6%的非肽聚糖多糖和49.9%的肽聚糖。
