Gallin J I, Rosenthal A S
J Cell Biol. 1974 Sep;62(3):594-609. doi: 10.1083/jcb.62.3.594.
Optimal human granulocyte chemotaxis has been shown to require both calcium and magnesium. Exposure of granulocytes to three different chemotactic factors (C5a, kallikrein, and dialyzable transfer factor) yielded a rapid calcium release, depressed calcium uptake, and was associated with a shift of calcium out of the cytoplasm and into a granule fraction. Colchicine, sodium azide, and cytochalasin B, in concentrations that inhibited chemotaxis, also inhibited calcium release while low concentrations of cytochalasin B, which enhanced chemotaxis, also enhanced calcium release. Microtubule assembly was visualized both in cells suspended in C5a without a chemotactic gradient and in cells actively migrating through a Micropore filter. The data suggest microtubule assembly is regulated, at least, in part, by the level of cytoplasmic calcium. It is proposed that asymmetric assembly of microtubules may be instrumental in imparting the net vector of motion during chemotaxis.
已证明最佳的人类粒细胞趋化性需要钙和镁。将粒细胞暴露于三种不同的趋化因子（C5a、激肽释放酶和可透析转移因子）会导致钙迅速释放、钙摄取降低，并伴随着钙从细胞质转移到颗粒部分。秋水仙碱、叠氮化钠和细胞松弛素B在抑制趋化性的浓度下也抑制钙释放，而低浓度的细胞松弛素B增强趋化性的同时也增强钙释放。在没有趋化梯度悬浮于C5a中的细胞以及通过微孔滤膜积极迁移的细胞中均可见微管组装。数据表明微管组装至少部分受细胞质钙水平的调节。有人提出微管的不对称组装可能有助于在趋化过程中赋予运动的净矢量。
