Bryant R E, DesPrez R M, VanWay M H, Rogers D E
J Exp Med. 1966 Sep 1;124(3):483-99. doi: 10.1084/jem.124.3.483.
Leukocyte migration was studied in a microhematocrit system which permitted evaluation of the separate effects of leukocyte adhesiveness, intrinsic cell motility, and leukocyte clumping. Leukocyte adhesion to glass required the presence of magnesium ion, was totally independent of calcium ion, and was partially dependent on heat-labile plasma factors. Leukocyte migration was unimpaired by marked acidosis or by wide variations in sodium, potassium, or calcium concentrations. Marked impairment of individual leukocyte motility and increased leukocyte aggregation were observed after phagocytosis of particulate matter, suggesting a mechanism facilitating recruitment and retention of leukocytes at areas of microbial invasion.
在微量血细胞比容系统中研究了白细胞迁移，该系统可评估白细胞黏附性、细胞内在运动性和白细胞聚集的单独作用。白细胞黏附于玻璃需要镁离子的存在，完全不依赖钙离子，部分依赖于热不稳定的血浆因子。显著的酸中毒或钠、钾或钙浓度的广泛变化不会损害白细胞迁移。吞噬颗粒物后观察到单个白细胞运动性显著受损和白细胞聚集增加，提示这是一种促进白细胞在微生物入侵部位募集和滞留的机制。
