Gallin J I, Durocher J R, Kaplan A P
J Clin Invest. 1975 May;55(5):967-74. doi: 10.1172/JCI108026.
The negative surface charge of human granulocytes was diminished after incubation with the chemotactic factors C5a, dialyzable transfer factor, and the enzymes kallikrein and plasminogen activator. No such change was observed after incubation with human IgG, albumin, horeseradish peroxidase, or a mixture of prekallikrein and plaminogen proactivator. Hydrocortisone inhibited the effect of C5a upon granulocyte surface charge and inhibited its chemotactic activity, suggesting that steroids act at the cell surface. The chemotactic inhibitors cholchicine and cytochalsin B had no effect upon granulocyte surface charge, consistent with their presumed effect upon microtubules and microfilaments, respectively. The data suggest that the decrease in cell surface charge may be a preerequiste for normal cell movement.
人粒细胞与趋化因子C5a、可透析转移因子、激肽释放酶及纤溶酶原激活剂孵育后，其表面负电荷减少。与人IgG、白蛋白、辣根过氧化物酶或前激肽释放酶与纤溶酶原激活剂混合物孵育后，未观察到这种变化。氢化可的松抑制C5a对粒细胞表面电荷的作用，并抑制其趋化活性，提示类固醇作用于细胞表面。趋化抑制剂秋水仙碱和细胞松弛素B对粒细胞表面电荷无影响，这分别与其对微管和微丝的推测作用一致。数据表明细胞表面电荷的减少可能是正常细胞运动的先决条件。
