网状内皮组织增殖症病毒在细胞培养中的复制：急性感染
Replication of reticuloendotheliosis viruses in cell culture: acute infection.
作者信息
Temin H M, Kassner V K
出版信息
J Virol. 1974 Feb;13(2):291-7. doi: 10.1128/JVI.13.2.291-297.1974.
Abstract
Replication of reticuloendotheliosis viruses (REV) in cultures of chicken and duck fibroblasts leads to some cell death soon after infection. This cell killing was used to develop a plaque assay for Trager duck spleen necrosis virus (TDSNV) on duck embryo fibroblasts. A normal replicative cell cycle was required for normal virus production and the development of cytopathic effects in chicken cells exposed to TDSNV. The latent period was about two days. Stationary chicken embryo fibroblasts could be infected by REV; DNA synthesis was required, but protein synthesis was not.
摘要
网状内皮组织增殖症病毒（REV）在鸡和鸭成纤维细胞培养物中复制时，感染后不久会导致一些细胞死亡。这种细胞杀伤作用被用于开发一种在鸭胚成纤维细胞上检测特拉格鸭脾坏死病毒（TDSNV）的蚀斑测定法。正常的病毒产生以及在暴露于TDSNV的鸡细胞中出现细胞病变效应需要正常的复制细胞周期。潜伏期约为两天。静止的鸡胚成纤维细胞可被REV感染；这需要DNA合成，但不需要蛋白质合成。
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