DNA-DNA反应的热色谱法。
Thermal chromatography of DNA-DNA reactions.
作者信息
Cowie D B, Szafranski P
出版信息
Biophys J. 1967 Sep;7(5):567-84. doi: 10.1016/S0006-3495(67)86607-4.
Abstract
Genetic relationships among bacteriophages and bacteria have been investigated using an extension of the DNA-agar technique. Thermal elution profiles, each characteristic of a specific DNA-DNA reaction, have been obtained from reactions involving homologous and heterologous DNAs. A sizable portion of the DNA of lysogenic (or semilysogenic DNA) viruses has been shown to be homologous to the DNA of their bacterial hosts. These homologies are contained in numerous sequences throughout the viral DNAs. Furthermore, DNA homologies exist among most temperate phage DNAs as well as with their respective hosts.
摘要
利用DNA-琼脂技术的扩展方法，对噬菌体和细菌之间的遗传关系进行了研究。从涉及同源和异源DNA的反应中获得了热洗脱图谱，每个图谱都是特定DNA-DNA反应的特征。已证明溶源性（或半溶源性DNA）病毒的相当一部分DNA与它们细菌宿主的DNA同源。这些同源性存在于整个病毒DNA的众多序列中。此外，大多数温和噬菌体DNA之间以及它们与各自宿主之间也存在DNA同源性。
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