Auling G, Bernard U, Hüttermann A, Mayer F
J Gen Virol. 1980 Jul;49(1):51-9. doi: 10.1099/0022-1317-49-1-51.
The double-stranded (ds)DNAs of the three closely related temperate Pseudomonas pseudoflava bacteriophages gd, ge and gf were studied biochemically and biophysically. The GC content of the DNA was 67.4 +/- 0.5% and differed only slightly from that of the host P. pseudoflava. By electron microscopic length measurements a mol. wt. of 26.1 X 10(6) to 26.7 X 10(6) was calculated for the three bacteriophage DNAs. Homogeneity of the bacteriophage DNAs was further demonstrated by specific cleavage with restriction endonucleases WcoRI and HindIII. It was concluded that the three homo-immune bacteriophages are identical. The genome size of the host P. pseudoflava GA3 was 3.7 X 10(9) as calculated from optical renaturation rate measurement with Xanthomonas pelargonii reference DNA. The bacteriophage gd genome thus amounts to 0.7% of the chromosome of this bacterial host.
对三种亲缘关系密切的温和型类黄假单胞菌噬菌体gd、ge和gf的双链（ds）DNA进行了生物化学和生物物理研究。DNA的GC含量为67.4±0.5%，与宿主类黄假单胞菌的GC含量仅略有差异。通过电子显微镜长度测量，计算出三种噬菌体DNA的分子量为26.1×10⁶至26.7×10⁶。限制性内切酶WcoRI和HindIII的特异性切割进一步证明了噬菌体DNA的均一性。得出结论，这三种同源免疫噬菌体是相同的。根据用天竺葵黄单胞菌参考DNA进行的光学复性速率测量，计算出宿主类黄假单胞菌GA3的基因组大小为3.7×10⁹。因此，噬菌体gd基因组占该细菌宿主染色体的0.7%。
