Vidal-Rioja L, Semorile L, Bianchi N O
Biochem Genet. 1979 Apr;17(3-4):297-303. doi: 10.1007/BF00498970.
Buoyant density in CsCl, melting temperature, and G + C base content of the DNA from four species of Akodon (Rodentia, Cricetidae) were determined. The buoyant density values of 1.699-1.701 g/cm3 were in accordance with the data reported for other cricetids. No satellite bands were seen in neutral CsCl. The Tm values determined in 1 x SSC ranged from 86.2 to 87.0 C, which corresponds to G + C contents of 41.2-43.2%. There was good agreement in DNA base composition of the four species, although values were slightly higher in A. obscurus, suggesting a certain degree of interspecies variability.
测定了四种阿氏鼠(啮齿目,仓鼠科)的DNA在氯化铯中的浮力密度、解链温度以及G + C碱基含量。1.699 - 1.701 g/cm³ 的浮力密度值与其他仓鼠科动物报道的数据一致。在中性氯化铯中未观察到卫星带。在1×SSC中测定的解链温度值范围为86.2至87.0℃,这对应于41.2 - 43.2%的G + C含量。这四个物种的DNA碱基组成有很好的一致性,尽管暗色阿氏鼠的值略高,表明存在一定程度的种间变异性。