对从绵羊基因组中获得的富含（dG + dC）的DNA片段进行物理分析。

Physical analysis of a (dG + dC)-rich fraction of DNA obtained from the ovine genome.

作者信息

Reisner A H, Colless V M, Bucholtz C A

出版信息

Aust J Biol Sci. 1980 Dec;33(6):623-32. doi: 10.1071/bi9800623.

Abstract

Using the organomercuric compound 3,6-bis(acetatomercurimethyl)dioxane in conjunction with Cs2SO4 density gradient equilibrium centrifugation a (dG + dC)-rich DNA fraction constituting 10% of the ovine genome was separated from the remainder. Further fractionation allowed four distinct classes of DNA to be identified with buoyant densities in neutral CsCl of 1.714, 1.717, 1.725 and 1.716 g/cm3. Computerized curve resolving of the data indicated the presence of several additional DNA classes. Data obtained using restriction endonuclease digestion indicated that the 1.714 g/cm3 satellite consists principally of an 820-base pair (bp) tandemly repeating unit. The 820-bp DNA is also present in the 1.717 g/cm3 satellites but as a minor component. The principal components of the 1.717 and 1.725 g/cm3 fractions are 125-, 176- and 235-bp fragments. In addition, these fractions contain 705-bp tandemly repeated material. Two or possibly three species of 22-bp tandem repeats were found only in the 1.725 g/cm3 DNA.

摘要

将有机汞化合物3,6 - 双（乙酰汞甲基）二氧六环与硫酸铯密度梯度平衡离心法相结合，从绵羊基因组的其余部分中分离出了占绵羊基因组10%的富含（dG + dC）的DNA组分。进一步分级分离后，鉴定出了四类不同的DNA，它们在中性氯化铯中的浮力密度分别为1.714、1.717、1.725和1.716 g/cm³。对数据进行计算机化曲线解析表明还存在其他几类DNA。使用限制性内切酶消化获得的数据表明，1.714 g/cm³的卫星DNA主要由一个820碱基对（bp）的串联重复单元组成。820 bp的DNA也存在于1.717 g/cm³的卫星DNA中，但为次要成分。1.717和1.725 g/cm³组分的主要成分是125、176和235 bp的片段。此外，这些组分还含有705 bp的串联重复物质。仅在1.725 g/cm³的DNA中发现了两种或可能三种22 bp的串联重复序列。