固定化酶移液器。一种简单装置的适用范围和局限性。
Immobilized-enzyme pipette. Scope and limitations of a simple device.
作者信息
Sundaram P V
出版信息
Biochem J. 1979 May 1;179(2):445-7. doi: 10.1042/bj1790445.
Abstract
Disposable pipette tips made of polymeric nylon tube with enzymes bound covalently to their inside surface and fixed to the stem of an automatic, adjustable-volume pipette holder together constitutes an immobilized enzyme pipette or 'Impette'. The present paper describes the application in research laboratories and clinics of this new development, with urease as an example in the determination of blood urea.
摘要
由聚合尼龙管制成的一次性移液器吸头，其内表面共价结合有酶，并固定在自动可调体积移液器架的杆上，共同构成了固定化酶移液器或“免疫移液器”。本文以脲酶为例，描述了这一新进展在研究实验室和临床中在测定血尿素方面的应用。
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