Smith R A
Biochem J. 1979 Jul 1;181(1):111-8. doi: 10.1042/bj1810111.
Amphipathic enzyme-polymer conjugates were prepared by reaction of hydrophilic enzymes with an anhydride polymer partially substituted with pendant hydrophobic groups. The products formed non-covalent aggregates of high molecular weight, dissociable by non-ionic detergents and urea and subject to additional aggregation at high ionic strength. Oil-in-water emulsions formed in the presence of such conjugates were shown to possess enzyme activity associated with the surface of the water-immiscible liquid. Fluorochrome labelling of conjugates showed that binding occurred at the surface of solvent droplets, and the conjugates were also found to aggregate liposomes.
通过使亲水性酶与部分被侧链疏水基团取代的酸酐聚合物反应制备两亲性酶-聚合物缀合物。所形成的产物形成高分子量的非共价聚集体，可被非离子洗涤剂和尿素解离，并在高离子强度下发生额外聚集。在这种缀合物存在下形成的水包油乳液显示具有与水不混溶液体表面相关的酶活性。缀合物的荧光染料标记表明结合发生在溶剂液滴表面，并且还发现缀合物可聚集脂质体。
