Sassen A, Vander Plaetse F, Janowski M, Maisin J R
Biomedicine. 1979 Jul;30(3):147-55.
The two main proteins p 30 and gp 70 from the Rauscher leukemia virus (RLV) have been isolated, labeled with 125I and used in radioimmune competition assays to estimate the amount of cross-reacting antigens produced during the evolution of various leukemias. The Rauscher leukemia in Balb/c mice, the radiation induced leukemia (RadLV) in C57Bl mice and two types derived therefrom by serial passages in mice (RadLV-RS) and in rats (RadLV-rat) were studied. Whereas the p 30 from RLV or RadLV (rat) viruses showed complete identity, the cross-reaction of their gp 70 proteins wasonly partial. The main findings in the tissues were the following: 1. The concentration of p 30 and gp 70 antigens increased much more during the RadLV-rat leukemia than in irradiated or RadLV (RS) treated mice. 2. In the serum, the ratio p 30/gp 70 was in general higher than one in the Rauscher leukemic mice and less than one in all types of RadLV leukemias. 3. In the spleen, RLV and RadLV (RS) infected mice had higher levels of p 30 than of gp 70. The reverse occurred in irradiated mice and in leukemic rats. Finally, the catabolic degradation of labeled RLV gp 70 was similar in normal and leukemic mice.
已从劳斯氏白血病病毒（RLV）中分离出两种主要蛋白质p30和gp70，用¹²⁵I进行标记，并用于放射免疫竞争分析，以估计各种白血病演变过程中产生的交叉反应抗原的量。对Balb/c小鼠的劳斯氏白血病、C57Bl小鼠的辐射诱导白血病（RadLV）以及通过在小鼠（RadLV-RS）和大鼠（RadLV-rat）中连续传代衍生出的两种类型进行了研究。虽然RLV或RadLV（大鼠）病毒的p30显示出完全相同，但它们的gp70蛋白的交叉反应只是部分的。组织中的主要发现如下：1. 在RadLV-大鼠白血病期间，p30和gp70抗原的浓度增加幅度远大于接受辐射或RadLV（RS）处理的小鼠。2. 在血清中，p30/gp70的比值在劳斯氏白血病小鼠中通常高于1，而在所有类型的RadLV白血病中均低于1。3. 在脾脏中，感染RLV和RadLV（RS）的小鼠p30水平高于gp70。在接受辐射的小鼠和白血病大鼠中则相反。最后，标记的RLV gp70在正常小鼠和白血病小鼠中的分解代谢降解情况相似。
