鼠伤寒沙门氏菌中的2-脱氧核糖基因-酶复合物。I. 2-脱氧核糖阴性突变体的分离与酶学特性分析
2-deoxyribose gene-enzyme complex in Salmonella typhimurium. I. Isolation and enzymatic characterization of 2-deoxyribose-negative mutants.
作者信息
Hoffee P A
出版信息
J Bacteriol. 1968 Feb;95(2):449-57. doi: 10.1128/jb.95.2.449-457.1968.
Abstract
Salmonella typhimurium was found to utilize 2-deoxyribose as a sole carbon and energy source. Cells grown in the presence of deoxyribose contained increased levels of deoxyribose kinase, thymidine phosphorylase, and two forms of deoxyribose-5-phosphate aldolase (DR5P aldolase). One form of DR5P aldolase was induced by deoxyribose and coordinately regulated with deoxyribose kinase. The second form of DR5P aldolase was induced by deoxyribose-5-phosphate and coordinately regulated with thymidine phosphorylase. Mutants unable to ferment deoxyribose have been isolated and shown to be lacking either deoxyribose kinase or deoxyribose permease, but none has been found from which DR5P aldolase is missing. Thymine-requiring mutants which are able to grow on low levels of thymine have been isolated and shown, in some cases, to be lacking one or both DR5P aldolases.
摘要
鼠伤寒沙门氏菌被发现能够利用2-脱氧核糖作为唯一的碳源和能源。在脱氧核糖存在的情况下生长的细胞中，脱氧核糖激酶、胸苷磷酸化酶以及两种形式的脱氧核糖-5-磷酸醛缩酶（DR5P醛缩酶）的水平有所增加。一种形式的DR5P醛缩酶由脱氧核糖诱导，并与脱氧核糖激酶协同调节。第二种形式的DR5P醛缩酶由脱氧核糖-5-磷酸诱导，并与胸苷磷酸化酶协同调节。已分离出无法发酵脱氧核糖的突变体，结果表明这些突变体要么缺乏脱氧核糖激酶，要么缺乏脱氧核糖通透酶，但尚未发现缺失DR5P醛缩酶的突变体。已分离出能够在低水平胸腺嘧啶上生长的需胸腺嘧啶突变体，在某些情况下，这些突变体被证明缺乏一种或两种DR5P醛缩酶。
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