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影响热处理大肠杆菌存活与复苏的因素

Factors influencing the survival and revival of heat-treated Escherichia coli.

作者信息

Russell A D, Harries D

出版信息

Appl Microbiol. 1968 Feb;16(2):335-9. doi: 10.1128/am.16.2.335-339.1968.

Abstract

Maximal revival of heat-damaged Escherichia coli occurred in nutrient media containing 0.8 to 1.0% (w/v) of Difco yeast extract. Vitamins did not appear to be involved in the recovery process. The situation with amino acids was less clear-cut, and, although certain of these may be essential for revival, proof for this is as yet inconclusive. Replica plating, in which colonies (from cells which had survived a heating process) on a rich medium were replicated onto minimal agar, revealed that no auxotrophic mutants had been formed as a result of heat treatment. Bacteria which were heated in 1% (w/v) yeast extract were killed more slowly than those heated in water.

摘要

在含有0.8%至1.0%(w/v)的Difco酵母提取物的营养培养基中,热损伤的大肠杆菌实现了最大程度的复苏。维生素似乎未参与复苏过程。氨基酸的情况则不太明确,尽管其中某些氨基酸可能对复苏至关重要,但对此的证据尚无定论。影印接种法是将丰富培养基上的菌落(来自经历过加热过程后存活的细胞)接种到基本琼脂上,结果显示热处理并未形成营养缺陷型突变体。在1%(w/v)酵母提取物中加热的细菌比在水中加热的细菌死亡得更慢。

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Revival of heat-damaged Escherichia coli.
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本文引用的文献

2
Replica plating and indirect selection of bacterial mutants.细菌突变体的影印培养和间接筛选
J Bacteriol. 1952 Mar;63(3):399-406. doi: 10.1128/jb.63.3.399-406.1952.
3
THERMAL INACTIVATION, HEAT INJURY, AND RECOVERY OF STAPHYLOCOCCUS AUREUS.
J Dairy Sci. 1965 Jun;48:677-81. doi: 10.3168/jds.s0022-0302(65)88321-7.
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The resistances of vegetative bacteria to moist heat.营养型细菌对湿热的抵抗力。
J Pharm Pharmacol. 1957 Dec;9(12):864-75; discussion 875-6. doi: 10.1111/j.2042-7158.1957.tb12348.x.
10
Revival of heat-damaged Escherichia coli.
Experientia. 1966 Dec 15;22(12):803-4. doi: 10.1007/BF01897426.

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