Russell A D, Harries D
Appl Microbiol. 1968 Feb;16(2):335-9. doi: 10.1128/am.16.2.335-339.1968.
Maximal revival of heat-damaged Escherichia coli occurred in nutrient media containing 0.8 to 1.0% (w/v) of Difco yeast extract. Vitamins did not appear to be involved in the recovery process. The situation with amino acids was less clear-cut, and, although certain of these may be essential for revival, proof for this is as yet inconclusive. Replica plating, in which colonies (from cells which had survived a heating process) on a rich medium were replicated onto minimal agar, revealed that no auxotrophic mutants had been formed as a result of heat treatment. Bacteria which were heated in 1% (w/v) yeast extract were killed more slowly than those heated in water.
在含有0.8%至1.0%(w/v)的Difco酵母提取物的营养培养基中,热损伤的大肠杆菌实现了最大程度的复苏。维生素似乎未参与复苏过程。氨基酸的情况则不太明确,尽管其中某些氨基酸可能对复苏至关重要,但对此的证据尚无定论。影印接种法是将丰富培养基上的菌落(来自经历过加热过程后存活的细胞)接种到基本琼脂上,结果显示热处理并未形成营养缺陷型突变体。在1%(w/v)酵母提取物中加热的细菌比在水中加热的细菌死亡得更慢。