辐照培养基对大肠杆菌修复缺陷菌株的毒性
Toxicity of irradiated medium for repair-deficient strains of Escherichia coli.
作者信息
Webb R B, Lorenz J R
出版信息
J Bacteriol. 1972 Oct;112(1):649-52. doi: 10.1128/jb.112.1.649-652.1972.
Abstract
Nutrient agar medium is made highly toxic to certain repair-deficient strains of Escherichia coli by exposure of the uninoculated plates to radiation from cool-white fluorescent lamps or black-light fluorescent lamps. This sensitivity is associated with the genetic deficiencies, fil, phr, and recA. Repair-sufficient and uvr strains are only slightly affected by the irradiated media. The poor growth and reduced plating efficiency frequently associated with BPhr and recA strains are very likely caused by inadvertent exposure of the medium to fluorescent light.
摘要
通过将未接种的平板暴露于冷白色荧光灯或黑光荧光灯的辐射下，营养琼脂培养基会对某些修复缺陷型大肠杆菌菌株产生高毒性。这种敏感性与fil、phr和recA的基因缺陷有关。修复充足的菌株和uvr菌株仅受到辐照培养基的轻微影响。与BPhr和recA菌株经常相关的生长不良和接种效率降低很可能是由于培养基意外暴露于荧光下所致。
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